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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | WB, FC | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABT189 |
| Description | Anti-Delta3 Antibody, clone 1E7.2 |
| Alternate Names |
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| Background Information | DLL3, also known as Delta like ligand 3, is a transmembrane Delta-like protein principally found in the Golgi apparatus and endosomes where it promotes Notch family receptor degradation to inhibit primary neurogenesis. DLL3 is one of five DSL proteins that bind to the Notch receptor. Notch signaling pathways play key roles in cell-fate determination and differentiation in many tissues during embryonic and postnatal development. Notch ligands family members are characterized by a DSL domain, EGF repeats, and a transmembrane domain. Mutations in the DLL3 gene causes autosomal recessive spondylocostal dysostosis. |
| Product Information | |
|---|---|
| Format | Purified |
| Control |
|
| Presentation | Purified mouse monoclonal IgMκ in buffer containing PBS with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This Anti-Delta3 Antibody, clone 1E7.2 is validated for use in WB, FC for the detection of Delta3. |
| Key Applications |
|
| Application Notes | Flow Cytometry Analysis: 0.1 µg from a representative lot detected Delta3 in HEK293 cells. |
| Biological Information | |
|---|---|
| Immunogen | Linear peptide corresponding to human Delta3. |
| Clone | 1E7.2 |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Mouse |
| Isotype | IgMκ |
| Species Reactivity |
|
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Entrez Gene Summary | This gene encodes a member of the delta protein ligand family. This family functions as Notch ligands that are characterized by a DSL domain, EGF repeats, and a transmembrane domain. Mutations in this gene cause autosomal recessive spondylocostal dysostosis 1. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Jul 2008]. |
| Gene Symbol |
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| UniProt Number |
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| UniProt Summary | FUNCTION: Inhibits primary neurogenesis. May be required to divert neurons along a specific differentiation pathway. Plays a role in the formation of somite boundaries during segmentation of the paraxial mesoderm (By similarity). SUBUNIT STRUCTURE: Can bind and activate Notch-1 or another Notch receptor (By similarity). SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein Probable. DOMAIN: The DSL domain is required for binding to the Notch receptor. PTM: Ubiquitinated by MIB (MIB1 or MIB2), leading to its endocytosis and subsequent degradation (By similarity). INVOLVEMENT IN DISEASE: Defects in DLL3 are the cause of spondylocostal dysostosis type 1 (SCDO1) [MIM:277300]. An autosomal recessive condition of variable severity associated with vertebral and rib segmentation defects. The main skeletal malformations include fusion of vertebrae, hemivertebrae, fusion of certain ribs, and other rib malformations. Deformity of the chest and spine (severe scoliosis, kyphoscoliosis and lordosis) is a natural consequence of the malformation and leads to a dwarf-like appearance. As the thorax is small, infants frequently have respiratory insufficiency and repeated respiratory infections resulting in life-threatening complications in the first year of life. SEQUENCE SIMILARITIES: Contains 1 DSL domain. Contains 6 EGF-like domains. |
| Molecular Weight | ~65 kDa observed |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blot in human liver tissue lysate. Western Blot Analysis: 0.5 µg/mL of this antibody detected Delta3 on 10 µg of human liver tissue lysate. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |