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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | WB | Rb | Unconjugated | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABT213 |
| Description | Anti-SHP-1 Antibody, clone HG213, rabbit monoclonal |
| Alternate Names |
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| Background Information | Src Homology region 2 domain Phosphatase 1 (SHP-1) is also known as Protein Tyrosine Phosphatase 1C (PTP1C), Protein Tyrosine Phosphatase non-receptor type 6 (PTPN6), and Hematopoietic Cell Phosphatase (HCPH). SHP-1 is a SH2 domain-containing cytosolic tyrosine phosphatase characterized by a catalytic phosphatase domain of approximately 240 amino acids that selectively dephosphorylates tyrosine residues in proteins; the SH2 domains are critical for SHP-1 to bind to ITIM domains in inhibitory immunoreceptors. SHP-1 is highly expressed in hematopoietic tissues and has a negative impact on lymphocyte signaling. SHP-1 is implicated in the control of tyrosine kinase signaling pathways in cellular proliferation, with a potential role in cancer. |
| Product Information | |
|---|---|
| Format | Unconjugated |
| Control |
|
| Presentation | Rabbit monoclonal supernatant without sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-SHP-1 Antibody, clone HG213 detects level of SHP-1 & has been published & validated for use in WB. |
| Key Applications |
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| Biological Information | |
|---|---|
| Immunogen | Full-length glutathione transferase SHP-1 fusion protein, purified from bacteria. |
| Clone | HG213 |
| Host | Rabbit |
| Species Reactivity |
|
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Entrez Gene Summary | The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. N-terminal part of this PTP contains two tandem Src homolog (SH2) domains, which act as protein phospho-tyrosine binding domains, and mediate the interaction of this PTP with its substrates. This PTP is expressed primarily in hematopoietic cells, and functions as an important regulator of multiple signaling pathways in hematopoietic cells. This PTP has been shown to interact with, and dephosphorylate a wide spectrum of phospho-proteins involved in hematopoietic cell signaling. Multiple alternatively spliced variants of this gene, which encode distinct isoforms, have been reported. |
| Gene Symbol |
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| Purification Method | Unpurified |
| UniProt Number |
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| UniProt Summary | FUNCTION: Plays a key role in hematopoiesis. This PTPase activity may directly link growth factor receptors and other signaling proteins through protein-tyrosine phosphorylation. The SH2 regions may interact with other cellular components to modulate its own phosphatase activity against interacting substrates. Together with MTUS1, induces UBE2V2 expression upon angiotensin II stimulation. SIZE: 595 amino acids; 67561 Da SUBUNIT: Monomer. Interacts with MTUS1 (By similarity). Binds PTPNS1, LILRB1 and LILRB2. Interacts with FCRL2, CD300LF and CD84. SUBCELLULAR LOCATION: Cytoplasm. Nucleus (By similarity). Note=In neurons, translocates into the nucleus after treatment with angiotensin II (By similarity). TISSUE SPECIFICITY: Isoform 1 is expressed in hematopoietic cells while isoform 2 is expressed in non-hematopoietic cells. PTM: Phosphorylated on serine and tyrosine residues. SIMILARITY: Belongs to the protein-tyrosine phosphatase family. Non-receptor class 2 subfamily. & Contains 2 SH2 domains. & Contains 1 tyrosine-protein phosphatase domain. |
| Molecular Weight | ~67 kDa observed. 3 isoforms exist with molecular weights between 63 kDa and 68 kDa. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blot in Jurkat cell lysate. Western Blot Analysis: A 1:1,000 dilution of this antibody detected SHP-1 in 10 µg of Jurkat cell lysate. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. |
| Packaging Information | |
|---|---|
| Material Size | 100 µL |