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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | WB, ICC, FC, IP, Inhibition, ACT | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABT398 |
| Description | Anti-CD226/DNAM-1 Antibody, clone LeoA1 (Azide Free) |
| Alternate Names |
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| Background Information | CD226/DNAM1 is a T cell expressed plasma membrane receptor protein that plays multiple roles in lymphocyte signaling, intercellular adhesion, lymphokine secretion and cytotoxic T-cell and NK cell mediated killing. CD226 activates natural killer (NK) cell-mediated cytotoxicity and is a key player in the immune synapse between killer T cells and targets. Also, because it recognizes CD155 and nectins CD226/DNAM1 is being examined as a potential anti-cancer agent because soluble CD226 directly inhibits tumor growth by binding CD155 expressed on tumor cells and down regulating proliferation. CD226/DNAM-1 is expressed on Th1, but not Th2 or Th0 cells, it is also expressed on NK cells, CD8+ T-cells, but not developing T cells. CD226/DNAM-1 is also expressed on monocytes and platelets. |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified mouse monoclonal IgG1κ in buffer containing PBS without preservatives. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This Anti-CD226/DNAM-1 Antibody, clone LeoA1 (Azide Free) is validated for use in Western Blotting and Immunocytochemistry and Flow Cytometry and Immunoprecipitation and Inhibition and Activity Assay for the detection of CD226/DNAM-1. |
| Key Applications |
|
| Application Notes | Immunocytochemistry Analysis: A 1:50 dilution from a representative lot detected CD226/DNAM-1 in CD226 transfected CHO cells and non-transfected CHO cells. Flow Cytometry Analysis: 1.0 µg of this antibody from a representative lot detected CD226/DNAM-1 in 1X10E6 CD226 transfected CHO cells. Activity Assay Analysis: A representative lot detected CD226/DNAM-1 which induces platelet aggregation (Scott, J.L., et al. (1989). Journal of Biol. Chem. 264(23):13475-13482). Competative Binding Assay Analysis: A representative lot detected CD226/DNAM-1 in the binding of this antibody to resting and pharmacologically activated PHA-induced blasts and Jurkat cells (Sherrington, P.D., et al. (1997). Journal of Biol. Chem. 272:21735-21744). Inhibition Assay Analysis: A representative lot detected CD226/DNAM-1 which inhibited the induction of both CTL andAK cells from their precursors (Burns, G.F., et al. (1985). J. Exp. Med. 161:1063-1078). Immunoprecipitation Analysis: A representative lot detected CD226/DNAM-1 in MLA-I44 cells (Burns, G.F., et al. (1985). J. Exp. Med. 161:1063-1078). Immunoprecipitation Analysis: A representative lot detected CD226/DNAM-1 in Jurkat PTA-1 transfectants stimulated with TPA (Ralston, K.J., et al. (2004). Journal of Biol. Chem. 279:33816-33828). |
| Biological Information | |
|---|---|
| Immunogen | Whole cells corresponding to human CD226/DNAM-1. |
| Clone | LeoA1 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Mouse |
| Specificity | MABT398 does not work by western blotting under reducing conditions (for this application please see MABT341). |
| Isotype | IgG1κ |
| Species Reactivity |
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| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Protein G Purified |
| UniProt Number |
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| Molecular Weight | ~39 kDa observed |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in CD226 transfected CHO cell lysate. Western Blotting Analysis: 0.5 µg/mL of this antibody detected CD226/DNAM-1 in 10 µg of CD226 transfected CHO cell lysate. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |