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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| M | FC | R | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABT78 |
| Replaces | CBL1308 |
| Description | Anti-mouse CD44 (H-CAM) Antibody, clone KM201 |
| Alternate Names |
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| Background Information | CD44, also known as H-CAM is a distinct adhesion molecule that, with its 13 known isoforms, comprises a family of transmembrane glycoproteins involved in a wide variety of significant biological processes. Members belonging to this family are involved in apoptosis, migration, metastasis, tumor progression, lymphocyte homing, and the attachment of hematopoietic cells to stromal cells. CD44 is the primary cell surface receptor for hyaluronic acid (HA), and is also a notable target for WNT signaling within the intestinal mucosa. Recent studies have used CD44 marker as a means to isolate different cancer stem cells (CSC) such as; colorectal CSC, pancreatic CSC, and prostate CSC. |
| Product Information | |
|---|---|
| Format | Purified |
| Control |
|
| Presentation | Purified rat monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine, pH 7.4, 150 mM NaCl, with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Detect mouse CD44 (H-CAM) using this Anti-mouse CD44 (H-CAM) Antibody, clone KM201 validated for use in FC. |
| Key Applications |
|
| Biological Information | |
|---|---|
| Immunogen | Mouse bone marrow-derived stromal cells |
| Epitope | Near the hyaluronate binding domain |
| Clone | KM201 |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Rat |
| Specificity | The antibody recognizes CD44 near the hyaluronate binding domain. |
| Isotype | IgG1κ |
| Species Reactivity |
|
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Protein G |
| UniProt Number |
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| UniProt Summary | FUNCTION: Main cell surface receptor for hyaluronate. Adhesion to mucosal high endothelial venule and to types I and VI collagen. Probably involved in matrix adhesion, lymphocyte activation and lymph node homing. SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein. PTM: N-glycosylated By similarity. O-glycosylated; contains chondroitin sulfate glycans which can be more or less sulfated (By similarity). Phosphorylated; activation of PKC results in the dephosphorylation of Ser-742 (constitutive phosphorylation site), and the phosphorylation of Ser-708 (By similarity). POLYMORPHISM: Two allelic forms of this glycoprotein, PGP-1.1 and PGP-1.2, have been reported. The expressed product is PGP-1.1 (Ly-24.1). SEQUENCE SIMILARITIES: Contains 1 Link domain. |
| Molecular Weight | 86 kDa calculated |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Flow Cytometry in mouse bone marrow cells. Flow Cytometry Analysis: 0.1 µg of this antibody detected CD44 in mouse bone marrow cells. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |