Special Offers
100% Performance Guaranteed
Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| R, M, B | IHC, WB | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABT84 |
| Description | Anti-Aggrecan Antibody, clone 1-C-6 |
| Alternate Names |
|
| Background Information | Aggregating chondroitin sulfate proteoglycan or aggrecan, is one of the most studied proteoglycans. Many individual monomers of aggrecan bind to hyaluronic acid to form an aggregate, the monomer is known as aggrecan. The aggregates are comprised of up to 100 monomers attached to a single chain of hyaluronic acid. The high content of negatively charged polysaccharide chains contributes to the osmotic swelling of cartilage and in combination with intact collagen fibers results in articular cartilage resistance to compressive forces and high tensile strength. Aggrecan is abundant in articular cartilage and compromises 10% of its dry weight. |
| Product Information | |
|---|---|
| Format | Purified |
| Control |
|
| Presentation | Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This Anti-Aggrecan Antibody, clone 1-C-6 is validated for use in IH, WB for the detection of Aggrecan. |
| Key Applications |
|
| Application Notes | Western Blot Analysis: 1 µg/mL from a representative lot detected Aggrecan on 10 µg of A1D4 protein fraction from Bovine tissue. |
| Biological Information | |
|---|---|
| Immunogen | Reduced and alkylated Aggrecan isolated from Swarm Rat Chondrosarcoma |
| Clone | 1-C-6 |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Mouse |
| Isotype | IgG1κ |
| Species Reactivity |
|
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
|
| Entrez Gene Summary | An extracellular matrix proteoglycan; cleaved by ADAMTS1 [RGD, Feb 2006]. |
| Gene Symbol |
|
| Purification Method | Protein G Purified |
| UniProt Number |
|
| UniProt Summary | FUNCTION: This proteoglycan is a major component of extracellular matrix of cartilagenous tissues. A major function of this protein is to resist compression in cartilage. It binds avidly to hyaluronic acid via an N-terminal globular region. May play a regulatory role in the matrix assembly of the cartilage. SUBUNIT STRUCTURE: Interacts with FBLN1 and COMP (By similarity). SUBCELLULAR LOCATION: Secreted › extracellular space › extracellular matrix (By similarity). DOMAIN: Two globular domains, G1 and G2, comprise the N-terminus of the proteoglycan, while another globular region, G3, makes up the C-terminus. G1 contains Link domains and thus consists of three disulfide-bonded loop structures designated as the A, B, B motifs. G2 is similar to G1. The keratan sulfate (KS) and the chondroitin sulfate (CS) attachment domains lie between G2 and G3. PTM: Contains mostly chondroitin sulfate, but also keratan sulfate chains, N-linked and O-linked oligosaccharides. SEQUENCE SIMILARITIES: Belongs to the aggrecan/versican proteoglycan family. Contains 1 C-type lectin domain. Contains 1 Ig-like V-type (immunoglobulin-like) domain. Contains 4 Link domains. Contains 1 Sushi (CCP/SCR) domain. |
| Molecular Weight | Various |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Immunohistochemistry in normal mouse cartilage and normal rat cartilage tissues. Immunohistochemistry Analysis: A 1:1,000 dilution of this antibody detected Aggrecan in normal mouse cartilage and normal rat cartilage tissues. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |