Takara Living Colors mCherry Monoclonal Antibody \100 uL

The Living Colors mCherry Monoclonal Antibody is Protein A-purified and was raised against mCherry. It recognizes mCherry, mOrange, mOrange2, mPlum, and mStrawberry, as well as C- and N-terminal fusions containing these fluorescent proteins.

The mCherry monoclonal antibody detects PAmCherry-N1 in the lysate of mammalian cells.HEK 293 cells were transiently transfected with pPAmCherry-N1. Cell lysates (corresponding to 30,000 cells) were prepared from HEK 293 cells transiently expressing PAmCherry-N1 (Lane 1) or a negative control (untransfected cells; Lane 2). Both lysates and a positive control (5 ng recombinant mCherry; Lane 3) were separated by SDS-PAGE and analyzed by Western blot using the mCherry monoclonal antibody at the recommended dilution of 1:1000.