Takara IFN-gamma GoStix™ Plus

IFN-γ GoStix Plus employ a lateral flow-based detection method and accompanying smartphone application to enable rapid quantification of human IFN-γ from cell culture supernatant or serum samples in only 10 minutes. Simply apply 20 µl of sample and 80 µl of chase buffer to a GoStix cassette, wait 10 minutes for test and control bands on the cassette to develop, and then scan the cassette using a mobile device running the GoStix Plus app to quantify the amount of IFN-γ present in the sample. The dynamic range and reproducibility of IFN-γ GoStix Plus are comparable to the performance of an ELISA, such that users can expect to obtain similar results in a fraction of the time.

CytokineGoStixPlus workflow.Apply 20 µl of sample to a GoStix lateral flow cassette followed by 80 µl of chase buffer. Wait 10 minutes for the test to run, then scan the cassette using the GoStix Plus smartphone app.

Comparison of T-cell activation methods using IFN-γGoStixPlus and ELISA-based methods.Human primary T cells were isolated from peripheral blood (PB) mononuclear cells (MNCs) using negative selection. T cells were subsequently activated with either CD3/CD28-coated beads, CD3/CD28 tetramer complexes, or PHA (10 μg/mL). Supernatant samples were collected at the indicated timepoints and assayed for IFN-γ protein using IFN-γ GoStix Plus or an IFN-γ ELISA. Assay results were converted into IU/mL values using NIBSC reference standard 82/587.

Quantitation of human TH1 cytokines usingGoStixPlus lateral flow assays.Purified, human primary CD3+ T cells were activated for 24 hours using tetrameric antibody complexes to cell-surface ligands CD3, CD28, and CD2. Cell culture supernatants were collected at the indicated timepoints post activation and analyzed using IL-2, IFN-γ, and TNF-α GoStix Plus assays and the accompanying GoStix Plus app.

Timelines associated with commonly used methods for measuring cytokines.GoStixPlus:a lateral flow-based quantitation method.FastELISA:measurement using targeted, preformulated ELISA reagents.Standard ELISA:measurement using standard direct or indirect sandwich assay.Bead array:multiparametric measurement by flow cytometry of bead-based, immunocapture of cytokines.Glass array:multianalyte measurement using quantitative, glass-slide multiplex ELISA microarray platform. Assay durations were taken from manufacturers’ protocols.