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Cell Signaling Phospho-Rb (Ser807/811) (D20b12) Xp Rabbit mAb (Alexa Fluor 555 Conjugate)
List Price
$483.79
Your Price
$483.79
Cell Signaling Phospho-Rb (Ser807/811) (D20b12) Xp Rabbit mAb (Alexa Fluor 555 Conjugate) - CSIG (Additional S&H or Hazmat Fees May Apply)
NETA PART:
CSIG-8957S
MFG.PART:
8957S
UNSPSC:
12352200
Manufacturer:
Cell Signaling
| Size | 100 µl (50 tests) |
| Reactivity | H M R Mk |
| Sensitivity | Endogenous |
| Source/Isotype | Rabbit IgG |
| Application/Dilution | {Immunofluorescence (Immunocytochemistry): 1:50, Flow Cytometry (Fixed/Permeabilized): 1:50} |
| Storage | Supplied in PBS (pH 7.2), less than 0.1% sodium azide and 2 mg/ml BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze. |
| Specificity/Sensitivity | Phospho-Rb (Ser807/811) (D20B12) XP® Rabbit mAb (Alexa Fluor® 555 Conjugate) recognizes endogenous levels of Rb protein only when phosphorylated at Ser807, Ser811, or at both sites. This antibody does not cross-react with Rb phosphorylated at Ser608. |
| Species Reactivity | Human, Mouse, Rat, Monkey |
| Source/Purification | Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser807/811 of human Rb protein. |
| Background | The retinoblastoma tumor suppressor protein Rb regulates cell proliferation by controlling progression through the restriction point within the G1-phase of the cell cycle (1). Rb has three functionally distinct binding domains and interacts with critical regulatory proteins including the E2F family of transcription factors, c-Abl tyrosine kinase, and proteins with a conserved LXCXE motif (2-4). Cell cycle-dependent phosphorylation by a CDK inhibits Rb target binding and allows cell cycle progression (5). Rb inactivation and subsequent cell cycle progression likely requires an initial phosphorylation by cyclin D-CDK4/6 followed by cyclin E-CDK2 phosphorylation (6). Specificity of different CDK/cyclin complexes has been observed in vitro (6-8) and cyclin D1 is required for Ser780 phosphorylation in vivo (9). |
| SKU | CSIG-8957S |
|---|---|
| Featured | No |
| Supplier Part Number | 8957S |
| UM | EA |
| UNSPSC | 12352200 |
| Manufacturer | Cell Signaling |
| MSDS URL | Click here |
| Temperature | +4C |
| CountryOfOrigin | United States |
| ProductLine | CSIG |
| Qty | 1 |
| MinOrderQty | 1 |
| Weight | 7.000000 |
| Lead Time | 5 |
| Hazardous | N |
| ACT Ecolabel | No |