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Millipore Anti-Histone Antibody, Pan, Clone F152.C25.Wjj Is A Mouse Monoclonal Antibody For Detection Of Histone Also Known As Histone H1.4, Histone H1b & Has Been Validated In Wb, Elisa. - Mill (Additional S&H or Hazmat Fees May Apply)
This antibody detects histone H1 and nuclesomal core proteins histones H2a, H2b, H3, H4. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Histone H1 is a nuclear protein necessary for condensation of nucleosome chains into higher order structures.
Product Information
Format
Purified
Control
Jurkat cell lysate
Presentation
Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality Level
MQ100
Applications
Application
Anti-Histone Antibody, Pan, clone F152.C25.WJJ is a Mouse Monoclonal Antibody for detection of Histone also known as Histone H1.4, Histone H1b & has been validated in WB, ELISA.
Key Applications
Western Blotting
ELISA
Application Notes
ELISA Analysis: A representative lot was used by an independent laboratory in ELISA. (Mizoguchi, E., et al. (1997). Gastroenterology. 113:1828-1835.)
Biological Information
Clone
F152C25W
Concentration
Please refer to the Certificate of Analysis for the lot-specific concentration.
Host
Mouse
Isotype
IgG2aκ
Species Reactivity
Human
Bovine
Antibody Type
Monoclonal Antibody
Gene Symbol
HIST1H1E
H1F4
Purification Method
Protein G
UniProt Number
P10412
Molecular Weight
~14-33 kDa observed
Product Usage Statements
Quality Assurance
Evaluated by Western Blot in Jurkat cell lysate.
Western Blot Analysis: 0.5 µg/mL of this antibody detected Histone H1 and core proteins on 10 µg of Jurkat cell lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
