Sigma-Aldrich Elastin Staining Solution

Vapor Pressure

59 hPa ( 20 °C)

Quality Level

400

Form

liquid

Expl. Lim.

3.5-15 % (v/v) ethanol)

Ivd

forin vitrodiagnostic use

Bp

78.3 °C/1013 hPa (ethanol)

Transition Temp

flash point 18 °C

Density

0.84 g/cm³at 20 °C

Application(S)

clinical testing
diagnostic assay manufacturing
hematology
histology

Storage Temp.

2-8°C

Related Categories

Hematology Stains

General Description

The ELASTIN staining solution acc. to Weigert - for microscopy, is a ready-to-use solution used to detect elastic fibers in connective tissues. Elastic connective-tissue fibers are bundles of glycoprotein-containing microfibrils that accumulate the polymeric protein elastin in their development, gradually resulting in the formation of coarse elastic fibers and membranes. The microfibrils on the surface of the elastic connective-tissue fibers expose high numbers of disulfide groups, which can be chemically detected when sufficient material is available for light-microscopic investigations. Furthermore, they are strongly acidic and thus bind the resorcin-fuchsin dye by electropolarity via interface adsorption.
The resorcin-fuchsin staining method is a regressive method. In tissue sections, nuclei can be stained using a 0.1% nuclear fast red-aluminum sulfate solution (Product number 1.00121) if not only elastic fibers, but also nuclei and the background also need to be visualized.
Brilliant staining results of these fibres are usually achieved when using the ELASTIN staining solution acc. to Weigert - for microscopy in combination with the Picrofuchsin solution acc. van Gieson - for microscopy, Product number 1.00199 and nuclear staining with Weigert′s iron hematoxylin kit - for nuclear staining in histology, Product number 1.15973 respectively Nuclear fast red-aluminium sulfate solution 0.1% - for microscopy, Product number 1.00121.
The 500ml bottle is sufficient for 400 - 500 applications. This product is registered as IVD and CE marked. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.

Analysis Note

Suitability for microscopy: passes test
Nuclei: blue-brown
Collagen: red
Muscle fibers: yellow
Cytoplasm: yellow
Elastic fibers: black

Pictograms

GHS02,GHS05

Signalword

Danger

Hcodes

H225 - H290 - H319

Pcodes

P210 - P233 - P234 - P240 - P241 - P305 + P351 + P338

Hazard Classifications

Eye Irrit. 2 - Flam. Liq. 2 - Met. Corr. 1

Storage Class

3 - Flammable liquids

Wgk_Germany

WGK 3

Flash_Point_F

64.4 °F

Flash_Point_C

18 °C