Sigma-Aldrich Listeria Selective Enrichm

Agency

(IDF-FIL)

Quality Level

200

Reg. Compliance

FDA -BAM

Form

solid

Composition

Acriflavine HCl, 5 mg per vial
Cycloheximide, 25 mg per vial
Nalidixic acid (sodium salt), 20 mg per vial

Packaging

pkg of 10 vials

Technique(S)

microbiological culture: suitable

Application(S)

food and beverages
microbiology

Compatibility

for use withListeria-Enrichment-Broth (Base)
for use with GranuCult^®BufferedListeriaEnrichment Broth (base)

Storage Temp.

2-8°C

Related Categories

Microbial Culture Media

Supplements for Cell Culture

General Description

Listeriaselective enrichment supplement is a mixture of two antibiotics and a dye in lyophilized form. It inhibits the growth of accompanying bacteria for the selective enrichment ofListeria monocytogenes.

Application

Listeriaselective enrichment supplement is an additive for the preparation ofListeriaenrichment broth (Base) and BufferedListeriaenrichment broth.

Compatibility

• For use with Listeria-Enrichment-Broth (Base) (1.11951)
• For use with GranuCult^®Buffered Listeria Enrichment Broth (base) (1.09628)

Linkage

Replaces: 1117810017

Analysis Note

Supplement tested in Listeria-Enrichment-Broth (Base) acc. to FDA/IDF-FIL art. no. 1.11951.
Growth
- Listeria monocytogenes ATCC 19118:
good to very good
- Listeria monocytogenes ATCC 13932 (WDCM 00021): good to very good
- Listeria monocytogenes ATCC 35152 (WDCM 00109): good to very good
- Listeria innocua ATCC 33090 (WDCM 00017): good to very good
- Staphylococcus aureus ATCC 25923 (WDCM 00034): none to very good
- Escherichia coli ATCC 25922 (WDCM 00013): none
Incubation: 24 - 48 hrs.; 30 °C; aerobic.GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, GermanyGHS05,GHS06,GHS08,GHS09H290 - H300 - H314 - H341 - H351 - H360D - H411P260 - P273 - P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338