Sigma-Aldrich Hemacolor(R) Rapid Stainin

Quality Level

400

Form

liquid

IVD

forin vitrodiagnostic use

pH

6.8 (20 °C in H₂O)

Density

1.01 g/cm³at 20 °C

Application(S)

clinical testing
diagnostic assay manufacturing
hematology
histology

Storage Temp.

15-25°C

Related Categories

Cytology Stains

Hematology Stains

General Description

Hemacolor^®Rapid staining of blood smear - Solution 3: colour reagent blue, is a ready-to-use solution used for human-medical cell diagnosis and serves the fixing of native blood and bone-marrow smears of human origin in hematological and clinico-cytological specimen materials, for example smears of whole blood and bone marrow. The staining with Hemacolor^®yields a staining result that corresponds to the Pappenheim stain, with predominantly magenta stained nuclei. This is based on the molecular interaction of the Eosin Y dye and a complex of Azur B with DNA. Both dyes assemble to an Eosin Y - Azur B-DNA complex and the intensity of the resulting stain depends on the content of Azur B and the ratio of Azur B : Eosin Y. Furthermore, the resulting stain can vary depending on the influence of fixation, staining times, pH-value of the solutions or buffer substances. By using pH 7.2 buffered solutions with the Hemacolor^®Rapid staining of blood smear solutions, a high stability of the stain and clean, precipitation-free staining results can be guaranteed.
The package is sufficient for 1250 - 5000 applications. The buffer tablets acc. to Weise pH 7.2 (Product number 1.09468) are also available in larger pack sizes, thus, they can be useful for higher demands.
The three solutions needed for staining are all available individually as:

Hemacolor^®Rapid staining of blood smear solution 1, fixing solution (Product number 1.11955)
Hemacolor^®Rapid staining of blood smear solution 2, colour reagent red (Product number 1.11956)
Hemacolor^®Rapid staining of blood smear solution 3, colour reagent blue(Product number 1.11957)

or they can be purchased as staining sets:

Hemacolor^®Rapid staining of blood smear (Product number 1.11674) 3 x 100 mL
Hemacolor^®Rapid staining of blood smear (Product number 1.11661) 3 x 500 mL

This product is registered as IVD and CE marked. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.HEMACOLOR is a registered trademark of Merck KGaA, Darmstadt, Germany