Sigma-Aldrich Hptlc Cellulose Pkg Of 25

Material

aluminum support
cellulose matrix

Quality Level

100

Feature

binder Organic Polymer
fluorescent indicator: no

Packaging

pkg of 25 sheets

Technique(S)

thin layer chromatography (TLC): suitable

Layer Thickness

70-110 μm

Sheet L × W

20 cm × 20 cm

Storage Temp.

2-30°C

Related Categories

Thin Layer Chromatography (TLC)

General Description

25 Aluminium sheets 20 x 20 cmCellulose plates are used to analyze polar substances. An organic sorbent, cellulose is perfect for separating hydrophilic substances by partition chromatography. Typical applications include the analysis of amino acids, carbohydrates, and phosphates as well as nucleic acid and nucleic acid derivatives. We offer cellulose plates in two grades: TLC for conventional and HPTLC for demanding, high-performance separations.

Linkage

Replaces: 16092-1; 16092

Analysis Note

Layer thickness: 70 - 110 µM
Deviation of layer thickness per plate: ≤ 30 µM
Aminoacid test
hRf-values
- L-serine, amino acid test:
20 - 30
- L-threonine, amino acid test: 30 - 40
- L-alanine, amino acid test: 35 - 45
- L-methionine, amino acid test: 55 - 65
- L-phenylalanine, amino acid test: 60 - 70
- L-leucine, amino acid test: 70 - 80
Typical value determined on a sheet
Eluent: 1-Butanol/ acetone/ acetic acid/ water (35/35/7/23 v/v/v/v) twice developed