Sigma-Aldrich Hy-Lite(R) Plus Pen Indust

Packaging

pkg of 20 tests (20 pens & 20 pipettes)

Quality Level

100

Manufacturer/Tradename

HY-LiTE^®

Application(S)

industrial hygiene

Compatibility

for use with HY-LITE^®

Detection Method

bioluminescent

Storage Temp.

2-8°C

Related Categories

Hygiene Monitoring Instruments, Samplers, Tests & Accessories

General Description

The HY-LiTE^®Plus pen is a ready prepared test designed for use in a HY-LiTE^®2/2A luminometers and consists of the following parts:

• A protection cap with integrated dilution solution.
• A white sampling stick (covered by the protection cap) for accurate sampling of the liquid to be tested. The stick is coated with an extractant, which releases ATP from cellular material. The stick also transfers the sample into the cuvette and in a later step opens the reagent chamber.
• A cuvette filled with test buffer for dilution, buffering and neutralization of the sample.
• A reagent chamber sealed with aluminum foil, containing freeze-dried and stabilized Luciferin/Luciferase mixture.

The test is based on firefly bioluminescence, using reagents especially formulated to compensate for high levels of inhibitory compounds, which are often encountered in complex organic aqueous matrices like paint emulsion, anti-corrosion coatings, metal working fluids etc. The test also includes a tube with dilution medium and a disposable pipette, enabling the user to perform a pre-dilution of the sample to further reduce inhibition. The dilution step is not mandatory, but it is recommended to perform at least a 1:3 sample dilution unless it has already been validated.

Application

The HY-LiTE^®Plus Pen is intended for detection of ATP in samples where higher than usual levels of inhibition are suspected, such as paints and coatings, Metal Working fluids, Hydraulic fluids, activated sludge (wastewater treatment) and similar.

Suitability

For detection of ATP in Industrial samples where higher than usual levels of inhibition are suspected

Analysis Note

packaging: conforms
Appearance: conforms
Performance test: conformsHY-LITE is a registered trademark of Merck KGaA, Darmstadt, Germany