Sigma-Aldrich Fmoc-Glu(Otbu)-(Dmb)Gly-Oh

Quality Level

200

Product Line

Novabiochem^®

Form

powder

Reaction Suitability

reaction type: Fmoc solid-phase peptide synthesis

Manufacturer/Tradename

Novabiochem^®

Application(S)

peptide synthesis

Functional Group

carboxylic acid

Storage Temp.

2-8°C

Related Categories

Amino Acids, Resins & Reagents for Peptide Synthesis

General Description

Fmoc-Aaa-(Dmb)Gly-OH dipeptides offer the same benefits as pseudoproline dipeptides such as prevention of aggregation and improvements in product quality but for peptide sequences containing Gly. They are extremely easy to use. Standard coupling methods like PyBOP^®/DIPEA or DIPCDI/HOBt can be used for their introduction. Removal of the Dmb group and regeneration of the glycine residue occurs during the course of standard TFA-mediated cleavage reaction.

Associated Protocols and Technical Articles
Cleavage and Deprotection Protocols for Fmoc SPPS
Overcoming Aspartimide Formation in Fmoc SPPS

Analysis Note

Color (visual): white to off white
Appearance of substance (visual): powder
Identity (IR): passes test
Optical rotation α 25/D (c=1 in methanol): -31.0 - -27.0 °
Purity (TLC(157A)): ≥ 98 %
Assay (HPLC, area%): ≥ 97.0 % (a/a)
Solubility (1 mmole in 2 ml DMF): clearly soluble
Assay (acidimetric): ≥ 90.0 %
Water (K. F.): ≤ 2.0 %
Ethyl acetate (HS-GC): ≤ 0.5 %
Acetate (IC): ≤ 0.02 %

To see the solvent systems used for TLC of Novabiochem^®products please click here.Novabiochem is a registered trademark of Merck KGaA, Darmstadt, GermanyPyBOP is a registered trademark of Merck KGaA, Darmstadt, Germany