Sigma-Aldrich Chromolith(R) Prep RP-18e

Product Name

Chromolith^®RP-18 endcapped, L × I.D. 100 mm x 25 mm Prep column

Material

PEEK column

Quality Level

100

Agency

suitable for USP L1

Product Line

Chromolith^®

Feature

endcapped

Packaging

1 ea of

Extent Of Labeling

18% Carbon loading

Parameter

100 bar pressure
45 °C max. temp.

Technique(S)

HPLC: suitable
LC/MS: suitable (Prep LC)
UHPLC-MS: suitable
UHPLC: suitable

Related Categories

HPLC Columns

General Description

Preparative HPLC involves much higher sample loading volumes than analytical chromatography. Consequently, greater sample throughput and separation speed are essential for optimal productivity. These criteria are best fulfilled by Chromolith^®Prep RP-18 endcapped columns. The combination of macro and mesopores maximizes separation efficiency and flow rate, while minimizing resistance.

The revolutionary, monolithic silica with its unique combination of macropores and mesopores enables:
—Rapid separations at very low column back-pressure
—High matrix tolerance, allowing time and cost savings with simplified workflows
—Cost savings due to extended column lifetimes

Learn more:
Brochure: Race through your Separations on Any System. Chromolith® Monolithic Silica HPLC Columns

Application

• Simultaneous determination of eight arginine-related metabolites in cellular extracts using liquid chromatography-tandem mass spectrometry.: This study explores the usage of the Chromolith^®RP-18 endcapped column for precise metabolic profiling, showcasing its effectiveness in analyzing arginine-related metabolites with high resolution and throughput (Amatya et Al., 2020).


• Rapid Determination of 30 Polyphenols in Tongmai Formula, a Combination of Puerariae Lobatae Radix, Salviae Miltiorrhizae Radix et Rhizoma, and Chuanxiong Rhizoma, via Liquid Chromatography-Tandem Mass Spectrometry.: The use of Chromolith^®RP-18 endcapped allowed for the fast and efficient separation of polyphenols in complex herbal formulations, illustrating its capabilities in herbal chemistry applications (Wang et Al., 2017).


• Simultaneous Liquid Chromatographic Determination of Ebastine with Two Sympathomimetic Drugs Using a Monolithic Column.: This research highlights the Chromolith^®RP-18 endcapped column′s ability to simultaneously analyze multiple compounds, thereby providing a method that improves the efficiency of pharmaceutical analyses (Ibrahim and Wahba, 2017).

Analysis Note

Pressure: ≤ 20 bar
Theoretical Plates (N/M) (Anthracene): ≥ 40000
Capacity factor (Anthracene): 3.0 - 4.5
Tailing (USP): 0.8 - 1.4 %
separation factor (anthracene/progesterone): 1.9 - 2.5Chromolith is a registered trademark of Merck KGaA, Darmstadt, Germany