Sigma-Aldrich Zic(R)-Hilic (3.5um 200 An

Product Name

SeQuant^®ZIC-HILIC^™(3.5µm,200Å) 150 x 0.3 mm, suitable for HPLC

Material

stainless steel column

Quality Level

100

Agency

suitable for USP L114

Product Line

SeQuant^®

Feature

endcapped: no

Parameter

400 bar pressure
70 °C max. temp.

Technique(S)

HPLC: suitable
LC/MS: suitable (Capillary LC)
mass spectrometry (MS): suitable (High)

Column L × I.D.

15 cm × 0.3 mm

Matrix

fully porous particle

Related Categories

HPLC Columns

General Description

SeQuant® ZIC®-HILIC Columns are designed to retain and separate all types of polar and hydrophilic compounds and for robust chromatography with high selectivity and reproducibility. The silica-based ZIC^®-HILIC sorbent has a bonded stationary phase consisting of a highly polar, permanent zwitterion. Separation selectivity is favored by the 1:1 zwitterion charge balance, which makes the ZIC^®-HILIC Column overall neutral, with weak, but important, ionic interactions. Tuning of the selectivity on the ZIC^®-HILIC Column during method development is facilitated by the pH-inpendent permanent zwitterion, ensuring that only the analytes (and not the column) are affected during eluent optimization.The Pore size of 200 Å makes this column perfectly suitable for the separation of larger polar molecules under HILIC conditions.The column dimension of 250 mm length x 2.1 mm ID is the optimal choice for the separation of complex samples using a UHPLC or optimized HPLC Instrument providing low dwell volumes.Thanks to their favorable retention and robust performance, SeQuant^®HILIC columns have become extremely popular in numerous laboratories around the world. For example, US FDA (United States Food and Drug Administration) has recommended SeQuant^®ZIC^®-HILIC for analysis of melamine and related substances. SeQuant^®HILIC columns excel in stability and low bleed. These features make them particularly suitable for LC-MS applications where high background can lead to signal suppression and interference with quantitative measurements while also increasing instrument wear. Their high stability makes these high-performance HILIC columns also attractive for traditional HPLC applications.Learn more about the Supelco HPLC Column portfolio and Method development:Brochure: HPLC and UHPLC Column Selection Guide Brochure: A Practical Guide to HPLC - Method Development Guide

Application

• Click postsynthesis of microporous organic network@silica composites:This study presents the creation of novel composites for mixed-mode chromatography, showcasing an innovative approach to chemical separation technologies, which includes hydrophilic interaction liquid chromatography (HILIC) (Sun HF et Al., 2023).


• Evaluation of Hold-Up Volume Determination Methods:This research evaluates different methods and markers in HILIC, critical for refining analytical methods in complex sample matrices such as pharmaceuticals, enhancing accuracy and reproducibility in chemical analysis (Redon L et Al., 2023).


• Determination of polyphenolic content in berry juices:Utilizing focusing-modulated comprehensive two-dimensional liquid chromatography, this study highlights the application of HILIC in food analysis, demonstrating its effectiveness in extracting detailed nutritional information (Arena K et Al., 2023).


• Simple and sensitive determination of sulfites in Chinese herbal teas:This paper employs ultra-high-performance liquid chromatography coupled with mass spectrometry, showing HILIC′s role in ensuring consumer safety by detecting potentially harmful additives in herbal products (Yang L et Al., 2022).

SeQuant is a registered trademark of Merck KGaA, Darmstadt, GermanyZIC is a registered trademark of Merck KGaA, Darmstadt, GermanyZIC-HILIC is a trademark of Merck KGaA, Darmstadt, Germany