Sigma-Aldrich Zic(R)-Hilic (3.5um 200 An

Material

stainless steel column

Quality Level

100

Agency

suitable for USP L114

Product Line

SeQuant^®

Feature

endcapped: no

Parameter

400 bar pressure
70 °C max. temp.

Technique(S)

HPLC: suitable
LC/MS: suitable (Capillary LC)
mass spectrometry (MS): suitable (High)

Column L × I.D.

15 cm × 1 mm

Matrix

fully porous particle

Matrix Active Group

ZIC-HILIC bonding phase

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HPLC Columns

General Description

SeQuant® ZIC®-HILIC Columns are designed to retain and separate all types of polar and hydrophilic compounds and for robust chromatography with high selectivity and reproducibility. The silica-based ZIC^®-HILIC sorbent has a bonded stationary phase consisting of a highly polar, permanent zwitterion. Separation selectivity is favored by the 1:1 zwitterion charge balance, which makes the ZIC^®-HILIC Column overall neutral, with weak, but important, ionic interactions. Tuning of the selectivity on the ZIC^®-HILIC Column during method development is facilitated by the pH-inpendent permanent zwitterion, ensuring that only the analytes (and not the column) are affected during eluent optimization.The Pore size of 200 Å makes this column perfectly suitable for the separation of larger polar molecules under HILIC conditions.The column dimension of 250 mm length x 2.1 mm ID is the optimal choice for the separation of complex samples using a UHPLC or optimized HPLC Instrument providing low dwell volumes.Thanks to their favorable retention and robust performance, SeQuant^®HILIC columns have become extremely popular in numerous laboratories around the world. For example US FDA (United States Food and Drug Administration) has recommended SeQuant^®ZIC^®-HILIC for analysis of melamine and related substances. SeQuant^®HILIC columns excel in stability and low bleed. These features make them particularly suitable for LC-MS applications where high background can lead to signal suppression and interference with quantitative measurements while also increasing instrument wear. Their high stability makes these high-performance HILIC columns also attractive for traditional HPLC applications.Learn more about the Supelco HPLC Column portfolio and Method development:Brochure: HPLC and UHPLC Column Selection Guide Brochure: A Practical Guide to HPLC - Method Development GuideSeQuant is a registered trademark of Merck KGaA, Darmstadt, GermanyZIC is a registered trademark of Merck KGaA, Darmstadt, GermanyZIC-HILIC is a trademark of Merck KGaA, Darmstadt, Germany