Sigma-Aldrich Chromolith(R) Performance

Product Name

Chromolith^®RP-18 endcapped, L × I.D. 100 mm x 3 mm HPLC column

Material

PEEK column

Quality Level

100

Agency

suitable for USP L1

Product Line

Chromolith^®

Feature

endcapped

Packaging

1 ea of

Extent Of Labeling

18% Carbon loading

Parameter

200 bar pressure
45 °C max. temp.

Technique(S)

HPLC: suitable
LC/MS: suitable
mass spectrometry (MS): suitable (High)

Related Categories

HPLC Columns

General Description

Chromolith® columns are made of monolithic silica with a characteristic bimodal pore structure.

Chromolith^®Performance RP-18 endcapped 100-3 columns have macropores with 2 μm diameter, giving a column efficiency exceeding 80,000 plates/meter. The mesopores are 13 nm (130 Å), the surface modification is octadecylsilane with full end-capping. Chromolith^®Performance RP-18 endcapped 100-3 columns fall under the USP L1 Classification. The column efficiency is comparable to a 5 μm particle size fully porous particulate column. Chromolith^®Performance RP-18 endcapped 100-3 columns are perfectly suitable for LC-MS applications.
The column dimension of 100 mm length and 3 mm I.D. is the best choice for fast separations of complex samples on any HPLC and UHPLC System.

The revolutionary, monolithic silica with its unique combination of macropores and mesopores enables:
—Rapid separations at very low column back-pressure
—High matrix tolerance, allowing time and cost savings with simplified workflows
—Cost-savings due to extended column lifetimes
—Connection of columns, flow gradients and the use of mobile phases with higher viscosity

The recommended Guard-columns are:
Chromolith^®RP-18 endcapped 5-3 guard cartridges (3 pieces) [1.52005] with the Chromolith^®Guard Cartridge Holder 5 mm x 3 mm [1.52004]

Holder and guard cartridges are also available as Guard cartridge set [1.52008]
The Chromolith^®column coupler can be used to significantly increase the separation efficiency of Chromolith^®columns by coupling several columns in series (up to 100,000 plates/per column). [1.51467]

Learn more:
Brochure: Race through your Separations on Any System. Chromolith® Monolithic Silica HPLC Columns

All Supelco HPLC Columns, including monolithic Chromolith^®columns, are fully compatible to all HPLC and UHPLC Instruments.
Supelco HPLC Products: The Best Choice for Any LC Instrument

Application

• Rapid Determination of 30 Polyphenols in Tongmai Formula:This study utilized the Chromolith^®RP-18 endcapped column in a liquid chromatography-tandem mass spectrometry setup to analyze 30 polyphenolic compounds in a traditional Chinese medicine formula, demonstrating its application in pharmaceutical analysis and quality control of complex herbal formulations (Wang et Al., 2017).


• Ultra-fast gradient LC method for omeprazole analysis using a monolithic column:The Chromolith^®RP-18 endcapped column was employed in an ultra-fast gradient liquid chromatography method for the analysis of omeprazole in enteric-coated pellets, highlighting its efficiency in pharmaceutical quality control and rapid method development (Borges et Al., 2010).

Analysis Note

Appearance: passes test
Pressure: ≤ 30 bar
stability testing: passes test
Performance (anthracene)
Theoretical Plates (N/M) (Anthracene): ≥ 80000
Capacity factor (Anthracene): 3.9 - 4.6
Tailing (USP): 0.86 - 1.30 %
Selectivity
separation factor (anthracene/progesterone): 2.35 - 2.65Chromolith is a registered trademark of Merck KGaA, Darmstadt, Germany