Sigma-Aldrich Chromolith(R) Semiprep RP

Product Name

Chromolith^®RP-18 endcapped, L × I.D. 100 mm x 10 mm SemiPrep column

Material

PEEK column

Quality Level

100

Agency

suitable for USP L1

Product Line

Chromolith^®

Feature

endcapped

Packaging

1 ea of

Extent Of Labeling

18% Carbon loading

Parameter

150 bar pressure
45 °C max. temp.

Technique(S)

HPLC: suitable (Semi Prep HPLC)
LC/MS: suitable

Related Categories

HPLC Columns

General Description

Chromolith^®SemiPrep RP-18 endcapped HPLC columns are ideally suited for direct scale-up from analytical to semiprep. This is because they offer faster sample throughput at a lower operating pressure compared to semi-prep columns packed with 5 μm particles. Chromolith^®SemiPrep 10 mm i.d. columns combine high separation speed with excellent performance. This makes them the perfect alternative to particulate columns of 10 mm i.d. (and even 21.2 mm i.d.). They have the same bimodal porous silica rod structure as Chromolith^®analytical columns with an internal diameter of 4.6 mm. Their macropores are 2 μm in diameter and the mesopores are 13 nm. This combination dramatically reduces separation time while increasing efficiency.

The revolutionary, monolithic silica with its unique combination of macropores and mesopores enables:
—Rapid separations at very low column back-pressure
—High matrix tolerance, allowing time and cost savings with simplified workflows
—Cost-savings due to extended column lifetimes

The recommended Guard-columns are:
Chromolith^®SemiPrep RP-18 endcapped 10-10 guard cartridges (3 pieces) [1.52036] with the Chromolith^®Guard Cartridge Holder 10 mm x 10 mm [1.52037]

Learn more:
Brochure: Race through your Separations on Any System. Chromolith® Monolithic Silica HPLC Columns

Application

• Simultaneous determination of eight arginine-related metabolites in cellular extracts using liquid chromatography-tandem mass spectrometry.: This study explores the usage of the Chromolith^®RP-18 endcapped column for precise metabolic profiling, showcasing its effectiveness in analyzing arginine-related metabolites with high resolution and throughput (Amatya et Al., 2020).


• Rapid Determination of 30 Polyphenols in Tongmai Formula, a Combination of Puerariae Lobatae Radix, Salviae Miltiorrhizae Radix et Rhizoma, and Chuanxiong Rhizoma, via Liquid Chromatography-Tandem Mass Spectrometry.: The use of Chromolith^®RP-18 endcapped allowed for the fast and efficient separation of polyphenols in complex herbal formulations, illustrating its capabilities in herbal chemistry applications (Wang et Al., 2017).


• Simultaneous Liquid Chromatographic Determination of Ebastine with Two Sympathomimetic Drugs Using a Monolithic Column.: This research highlights the Chromolith^®RP-18 endcapped column′s ability to simultaneously analyze multiple compounds, thereby providing a method that improves the efficiency of pharmaceutical analyses (Ibrahim and Wahba, 2017).

Analysis Note

Carbon content: 18.0 - 22.0 %
Pressure: ≤ 30 bar
Performance (anthracene)
Theoretical Plates (N/M) (Anthracene): ≥ 80000
Capacity factor (Anthracene): 4.3 - 5.0
Tailing (USP): 0.90 - 1.45 %
Selectivity
separation factor (anthracene/progesterone): 1.95 - 2.35Chromolith is a registered trademark of Merck KGaA, Darmstadt, Germany