Sigma-Aldrich Purospher(R) Star RP-18 En

Product Name

Purospher^®STAR RP-18 endcapped (3 µM), L × I.D. 250 mm x 4.6 mm Hibar^®RT HPLC column

Material

stainless steel column

Quality Level

100

Agency

suitable for USP L1

Product Line

Purospher^®

Feature

endcapped

Packaging

1 ea of

Extent Of Labeling

17% Carbon loading

Parameter

400 bar pressure
65 °C max. temp.

Technique(S)

HPLC: suitable
LC/MS: suitable
mass spectrometry (MS): suitable (High)

Related Categories

HPLC Columns

General Description

Purospher® STAR RP-18 endcapped, 3 µM HPLC columns are designed for universal use and allow the separation of basic, neutral, and metal chelating compounds with simple mobile phases and excellent peak symmetry. They are based on ultra-pure fully porous silica particles (Type B). These columns offer an outstanding stability from pH 1.5 to 10.5 over a wide temperature range, and suitability for up to 100% aqueous mobile phases. Plus, they demonstrate best all-round retention characteristics, as proven by the Tanaka test. Thanks to these features, Purospher^®STAR RP-18 endcapped, 3 µM HPLC columns allow maximum flexibility to use the best chromatographic conditions for any separation including reversed phase.Purospher^®STAR RP-18 endcapped, 3 µM Hibar^®RT 250-4.6 is a ready to use stainless steel column suitable for connection to any HPLC system.The column dimension of 250 mm length x 4.6 mm I.D. is the optimal choice for high resolution HPLC separation of complex samples.The recommended guard column is Purospher^®STAR RP-18 endcapped (5 µM) LiChroCART^®4-4 part number 1.50250.0001. For direct connection to a Hibar^®RT columns the Precolumn holder for 4-4 LiChroCART^®cartridges, part number 1.51487.0001 can be used.Item 1.50471.1003 is a Validation kit containing 3 Purospher^®STAR RP-18 endcapped, 3 µM Hibar^®RT 250-4.6 HPLC columns from 3 different base material lots.Learn more about Purospher® STAR HPLC and UHPLC ColumnsLearn more about the Supelco HPLC Column portfolio and Method development:Brochure: HPLC and UHPLC Column Selection GuideBrochure: A Practical Guide to HPLC - Method Development Guide

Application

Intended to be used in the separation of aflatoxins, aldehydes, alkaloids, antibiotics, catecholamines, explosives, oligonucleotides, esters, fat soluble vitamins, lipids, fatty acids, flavonoids, ketones, nitrosamines, nucleosides, PAHs, PCBs, peptides, pesticides, phenols, phthalates, preservatives, steroids, metabolized steroids, sulfonamides, sweeteners, and water soluble vitamins.

Features And Benefits

• Ultra-pure silica (99.999%) for excellent peak symmetry
• High separation efficiency
• Reproducible results from run-to-run and batch-to-batch
• Best all-round performance according to Tanaka
• Outstanding pH stability from pH 1.5 to 10.5
• No phase collapse when using highly aqueous mobile phases
• Good suitability for LC-MS applications

Analysis Note

Pressure: ≤ 280 bar
Theoretical Plates (N/M) (Anthracene): ≥ 120000
Symmetry (Anthracene): 0.8 - 1.4
Capacity factor (Anthracene): 4.0 - 6.0Hibar is a registered trademark of Merck KGaA, Darmstadt, GermanyLICHROCART is a registered trademark of Merck KGaA, Darmstadt, GermanyPurospher is a registered trademark of Merck KGaA, Darmstadt, Germany