Sigma-Aldrich Lichrospher(R) RP-Select B

Product Name

LiChrospher^®RP-select B (5µm) Hibar^®RT 250-4.6, suitable for HPLC

Material

stainless steel column

Quality Level

100

Agency

suitable for USP L7

Product Line

LiChrospher^®

Feature

endcapped: no

Extent Of Labeling

11.5% Carbon loading

Parameter

400 bar pressure
60 °C max. temp.

Technique(S)

HPLC: suitable
mass spectrometry (MS): suitable (Low - Medium)

Column L × I.D.

25 cm × 4.6 mm

Related Categories

HPLC Columns

General Description

HPLC columnLiChrospher^®RP-select B is a versatile traditionally produced spherical silica gel carriers with reversed phase properties and with the hydrophobicity of a C-8 phase. This carrier is very well suited for the separation of basic compounds, but with still good properties for the determination of neutral and acidic compounds. This sorbent is packed into a Hibar 100-4.6 HPLC column. For mounting of a LiChroCART 4-4 guard column, the guard column holder 1.51487 has to be used.

Application

• Simultaneous quantification of delta-9-THC, THC-acid A, CBN and CBD in seized drugs using HPLC-DAD:This study utilizes high-performance liquid chromatography with diode-array detection (HPLC-DAD), employing a LiChrospher^®RP-select B column to effectively separate and quantify cannabinoids in forensic samples, highlighting its application in legal and forensic settings (Ambach et Al., 2014).


• Structural characterization of stress degradation products of Darunavir:Employing LC-MS/MS alongside HPLC, this research underscores the utility of the LiChrospher^®RP-select B column in the stability analysis of HIV medications, crucial for ensuring drug safety and efficacy (Rao et Al., 2014).


• RP-HPLC separation and characterization of unknown impurities of Darunavir:This study demonstrates the column′s role in identifying and quantifying impurities in HIV-protease inhibitors, contributing to the pharmaceutical industry′s quality control measures (Nageswara Rao et Al., 2013).


• Quantification of pralidoxime in urine by ion pair chromatography:The LiChrospher^®RP-select B column is applied in a biomedical context to monitor therapeutic agents in clinical settings, supporting patient treatment regimes (John et Al., 2012).


• Liquid chromatography/electrospray tandem mass spectrometry method for determination of cefuroxime:This analytical method facilitates the study of pharmacokinetics in clinical pharmacology, using the LiChrospher^®RP-select B column for precise drug monitoring in plasma (Partani et Al., 2010).

Analysis Note

Pressure: ≤ 90 bar
Theoretical Plates (N/M) (Toluene): ≥ 50000
Symmetry (Toluene): 0.8 - 1.4
Capacity factor (Toluene): 1.0 - 2.0Hibar is a registered trademark of Merck KGaA, Darmstadt, GermanyLiChrospher is a registered trademark of Merck KGaA, Darmstadt, Germany