Sigma-Aldrich Chromolith(R) Flash RP-18

Product Name

Chromolith^®RP-18 endcapped, L × I.D. 25 mm x 2 mm HPLC column

Material

PEEK column

Quality Level

100

Agency

suitable for USP L1

Product Line

Chromolith^®

Feature

endcapped

Packaging

1 ea of

Extent Of Labeling

18% Carbon loading

Parameter

200 bar pressure
45 °C max. temp.

Technique(S)

HPLC: suitable
LC/MS: suitable
UHPLC-MS: suitable
UHPLC: suitable
mass spectrometry (MS): suitable (High)

Related Categories

HPLC Columns

General Description

Chromolith® columns are made of monolithic silica with a characteristic bimodal pore structure.

Chromolith^®Flash RP-18 endcapped 25-2 columns have macropores with 1.5 μm diameter, giving a column efficiency exceeding 100,000 plates/meter. The mesopores are 13 nm (130 Å), the surface modification is octadecylsilane with full end-capping. Chromolith^®Flash RP-18 endcapped 25-2 columns fall under the USP L1 Classification. The column efficiency is comparable to a 3-4 μm particle size fully porous particulate column. Chromolith^®Flash RP-18 endcapped 25-2 columns are perfectly suitable for LC-MS applications.
The column dimension of 25 mm length and 2 mm I.D. is the best choice for rapid separations on any U/HPLC System.

The revolutionary, monolithic silica with its unique combination of macropores and mesopores enables:
—Rapid separations at very low column back-pressure
—High matrix tolerance, allowing time and cost savings with simplified workflows
—Cost-savings due to extended column lifetimes
—Connection of columns, flow gradients and the use of mobile phases with higher viscosity

The recommended Guard-columns are:
Chromolith^®RP-18 endcapped 5-2 guard cartridges (3 pieces) " /product/mm/152009">1.52009] with the Chromolith^®Guard Cartridge Holder (kit) 5 mm x 2 mm [1.52004]
The Chromolith^®column coupler can be used to significantly increase the separation efficiency of Chromolith^®columns by coupling several columns in series (up to 100,000 plates/per column). [1.51467]

Learn more:
Brochure: Race through your Separations on Any System. Chromolith® Monolithic Silica HPLC Columns

All Supelco HPLC Columns, including monolithic Chromolith^®columns, are fully compatible to all HPLC and UHPLC Instruments.
Supelco HPLC Products: The Best Choice for Any LC Instrument

Analysis Note

Pressure: ≤ 20 bar
Performance (anthracene)
Theoretical Plates (N/M) (Anthracene): ≥ 70000
Capacity factor (Anthracene): 3.3 - 4.3
Tailing (USP): 0.90 - 1.40 %
separation factor (anthracene/progesterone): 2.2 - 2.6Chromolith is a registered trademark of Merck KGaA, Darmstadt, Germany