Sigma-Aldrich Chromolith(R) Wp 300 Prote

Product Name

Chromolith^®WP 300 Protein A, L × I.D. 25 mm x 4.6 mm HPLC column

Material

PEEK column

Product Line

Chromolith^®

Feature

endcapped: no
endcapped: no

Packaging

1 ea of

Parameter

2 - 45 °C temp. range
2-45 °C temp. range
200 bar pressure
200 bar pressure

Technique(S)

HPLC: suitable
HPLC: suitable
LC/MS: suitable

Column L × I.D.

2.5 cm × 4.6 mm

Surface Area

120 M²/g

Matrix

monolithic silica
monolithic silica

Related Categories

HPLC Columns

General Description

Affinity chromatography is a selective technique which takes advantage of specific molecular interactions, for example antigen and antibody. The Chromolith^®WP 300 Protein A HPLC column is designed to monitor monoclonal antibody titer and yield determination from cell-culture supernatants. This analytical scale procedure helps to optimize the titer of monoclonal antibody for the optimal time for harvest of the monoclonal antibody products.

Chromolith^®WP 300 Protein A 25-4.6 columns are made of monolithic silica with a characteristic bimodal pore structure.

Key Benefits:
—Rapid and accurate titer determination across a wide range of injection volumes
—Consistent binding efficiencies even at high flow rates
—Expanded column lifetimes

Learn more:
Brochure: Race through your Separations on Any System. Chromolith® Monolithic Silica HPLC Columns

Chromolith^®WP 300 Protein A is available in 2 mm I.D. providing improved efficiency, sensitivity, and enhanced MS-compatibility.
Flyer: Rapid and Robust Antibody Quantitation - Chromolith® WP 300 Protein A HPLC columns

All Supelco HPLC Columns, including monolithic Chromolith^®columns, are fully compatible to all HPLC and UHPLC Instruments.
Supelco HPLC Products: The Best Choice for Any LC InstrumentChromolith is a registered trademark of Merck KGaA, Darmstadt, Germany