Sigma-Aldrich Mir-372/3 Inhibitor, Neomy

Assay

≥98% (HPLC)

Quality Level

100

Form

solid

Manufacturer/Tradename

Calbiochem^®

Storage Condition

OK to freeze
desiccated (hygroscopic)
protect from light

Color

off-white

Solubility

DMSO: 100 mg/mL

Storage Temp.

−70°C

General Description

A cell-permeable neomycin derived artificial nucleobase conjugate that inhibits the production of oncogenic miRNAs 372 (IC₅₀= 2.4 µM; K_d= 16 nM) and 373 (IC₅₀= 5.13 µM K_d= 21.4 nM) by directly binding to their precursors pre-miRNAs and interrupts their processing by Dicer. In comparison, neomycin does not affect these miRNAs (IC₅₀= 125 µM; K_d= 7.4 µM). Shown to depress proliferation of AGS gastric cancer cells expressing miRNA 372 and 373. However, it has no effect on MKN74 gastric epithelial cells that do not express miRNA 372 and 373. Reduced levels of miRNA 372 results in de-repression of their targets, such as the cell cycle regulator large tumor suppressor homologue 2 (LATS2).A cell-permeable neomycin derived artificial nucleobase conjugate that inhibits the production of oncogenic miRNAs 372 (IC₅₀= 2.4 µM; K_d= 16 nM) and 373 (IC₅₀= 5.13 µM K_d= 21.4 nM) by directly binding to their precursors pre-miRNAs and interrupts their processing by Dicer. In comparison, neomycin does not affect these miRNAs (IC₅₀= 125 µM; K_d= 7.4 µM). Shown to depress proliferation of AGS gastric cancer cells expressing miRNA 372 and 373. However, it has no effect on MKN74 gastric epithelial cells that do not express miRNA 372 and 373. Reduced levels of miRNA 372 results in de-repression of their targets, such as the cell cycle regulator large tumor suppressor homologue 2 (LATS2).

Please note that the molecular weight for this compound is batch-specific due to variable water content.

Biochem/Physiol Actions

Primary Target
miRNA-272/273Reversible: yes

Packaging

Packaged under inert gas

Warning

Toxicity: Standard Handling (A)

Reconstitution

Following reconstitution, aliquot and freeze (-2-°C). Stock solutions are stable for up to 3 months at -20°C.

Other Notes

Vo, D.D., et Al. 2014.ACS Chem. Biol. 9,711.CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany