Sigma-Aldrich Pi4kiiibeta Inhibitor, Bf7

Assay

≥98% (HPLC)

Quality Level

100

Form

powder

Potency

5.7 nM IC₅₀

Manufacturer/Tradename

Calbiochem^®

Storage Condition

OK to freeze
protect from light

Color

light gray

Solubility

DMSO: 50 mg/mL

Storage Temp.

2-8°C

General Description

A cell-permeable imidazo-pyrazine based compound that acts as a potent and reversible inhibitor of PI4KIIIβ (IC₅₀= 5.7 nM). Displays excellent selectivity over PI4KIIIα (IC₅₀= 1.7 µM) and several other lipid kinases (IC₅₀>10 µM). Shown to dose-dependently decrease PI4P levels, reduce oxysterol-binding protein (OSBP) localization to replication organelles (ROs) and inhibit cholesterol shuttling to ROs (at 1 µM in BGM and HeLaR19 cells transfected with CVB3) at multiplicity of infection (MOI). Exhibits uniformly potent and broad spectrum antienteroviral activity across a wide range of species (EC₅₀= 4-31 nM) and is inactive against RNA, DNA, and retroviruses.

Biochem/Physiol Actions

Cell permeable: yesPrimary Target
PI4KIII&betaReversible: yes

Packaging

Packaged under inert gas

Warning

Toxicity: Standard Handling (A)

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Other Notes

Strating, J.R.P.M., et Al. 2015.Cell Reports. 10,600.
MacLeod, A.M., et Al. 2013.ACS Med. Chem. Lett. 4,585.CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany