Sigma-Aldrich Fmoc-Tyr(PO(Nme₂)&#8

Quality Level

200

Product Line

Novabiochem^®

Assay

≥93.0% (acidimetric)
≥96% (TLC)
≥96.0% (HPLC)

Form

powder

Reaction Suitability

reaction type: Fmoc solid-phase peptide synthesis

Manufacturer/Tradename

Novabiochem^®

Application(S)

peptide synthesis

Functional Group

Fmoc

Storage Temp.

15-25°C

Related Categories

Amino Acids, Resins & Reagents for Peptide Synthesis

General Description

Fmoc-Tyr(PO(NMe₂)₂)-OH is a useful derivative for the synthesis of phosphotyrosine-containing peptides by Fmoc SPPS . As the side-chain phosphate group is fully protected, this derivative is compatible with all coupling methods.Regeneration of phosphotyrosine from the phosphodiamidate is effected by acid catalyzed hydrolysis using 90% aq. TFA.

Associated Protocols and Technical Articles
Cleavage and Deprotection Protocols for Fmoc SPPS

Literature references

H. g. Chao, et Al. (1995)J. Org. Chem.,60, 7710.

Linkage

Replaces: 04-12-1205

Analysis Note

Color (visual): white to slight yellow to beige
Appearance of substance (visual): powder
Identity (IR): passes test
Enantiomeric purity: ≥ 99.5 % (a/a)
Purity (TLC(0811)): ≥ 98 %
Assay (HPLC, area%): ≥ 96.0 % (a/a)
Solubility (1 mmole in 2 mL DMF): clearly soluble
Assay (acidimetric): ≥ 93.0 %
Water (K. F.): ≤ 4.5 %
Ethyl acetate (HS-GC): ≤ 0.5 %
Acetate (IC): ≤ 0.1 %

To see the solvent systems used for TLC of Novabiochem^®products please click here.Novabiochem is a registered trademark of Merck KGaA, Darmstadt, Germany