Sigma-Aldrich Fmoc-Lys(Mca)-Oh Novabioch

Quality Level

200

Product Line

Novabiochem^®

Assay

≥97% (TLC)
≥97.0% (HPLC)

Form

powder

Reaction Suitability

reaction type: Fmoc solid-phase peptide synthesis

Manufacturer/Tradename

Novabiochem^®

Application(S)

peptide synthesis

Functional Group

amine

Storage Temp.

15-25°C

Inchi

1S/C33H32N2O8/c1-41-21-13-14-22-20(17-31(37)43-29(22)18-21)16-30(36)34-15-7-6-12-28(32(38)39)35-33(40)42-19-27-25-10-4-2-8-23(25)24-9-3-5-11-26(24)27/h2-5,8-11,13-14,17-18,27-28H,6-7,12,15-16,19H2,1H3,(H,34,36)(H,35,40)(H,38,39)/t28-/m0/s1

Related Categories

Amino Acids, Resins & Reagents for Peptide Synthesis

General Description

A modified lysine derivative for the preparation of fluorogenically-labeled peptides by Fmoc SPPS . The Mca group fluoresces at 405 nm when stimulated at 340 nm, and is most commonly used in conjunction with Dabcyl and 2,4-dinitrophenyl quenching groups. Recently, Fmoc-Lys(Mca)-OH has been used to prepare lanthanide-based luminescent proteins by native chemical ligation . Lys(Mca) was found to be stable to HF cleavage conditions used to synthesize the thioester fragments needed for ligation.

Associated Protocols and Technical Articles
Cleavage and Deprotection Protocols for Fmoc SPPS
Chromogenic Labeling of Synthetic Peptides

Literature references

J. L. Lauer-Fields, et Al. (2001)Biochemistry,40, 5795.
C. F. W. Becker, et Al. (2004)Bioconjugate Chem.,15, 1118.

Linkage

Replaces: 04-12-1233

Analysis Note

Color (visual): white to slight yellow to beige
Appearance of substance (visual): powder
Identity (IR): passes test
Enantiomeric purity: ≥ 99.0 % (a/a)
Purity (TLC(011A)): ≥ 97 %
Assay (HPLC, area%): ≥ 97.0 % (a/a)
Water (K. F.): ≤ 2.0 %

To see the solvent systems used for TLC of Novabiochem^®products please click here.Novabiochem is a registered trademark of Merck KGaA, Darmstadt, Germany