Sigma-Aldrich Fmoc-Lys(Me,Boc)-Oh Novabi

Quality Level

200

Product Line

Novabiochem^®

Assay

≥97.0% (HPLC)
≥98% (TLC)

Form

powder

Reaction Suitability

reaction type: Fmoc solid-phase peptide synthesis

Manufacturer/Tradename

Novabiochem^®

Application(S)

peptide synthesis

Functional Group

amine

Storage Temp.

2-8°C

Inchi

1S/C27H34N2O6/c1-27(2,3)35-26(33)29(4)16-10-9-15-23(24(30)31)28-25(32)34-17-22-20-13-7-5-11-18(20)19-12-6-8-14-21(19)22/h5-8,11-14,22-23H,9-10,15-17H2,1-4H3,(H,28,32)(H,30,31)/t23-/m0/s1

Related Categories

Amino Acids, Resins & Reagents for Peptide Synthesis

General Description

A novel derivative for the introduction of monomethyl-lysine during Fmoc SPPS ^[1,2] . Coupling can be carried out using any standard activation method. Removal of the Boc protecting group occurs during the course of the TFA-mediated cleavage reaction. Ref contains methods and protocols for the synthesis of arrays of histone-related peptides containing methylated arginine and lysine-residues.

Associated Protocols and Technical Articles
Cleavage and Deprotection Protocols for Fmoc SPPS
Overcoming Aggregation in Fmoc SPPS

Literature references

A.J. Bannister (2002)Cell,109, 801.
A.E. McBride & P.A. Silver (2001)Cell,106, 5.
S. Rothbart, et Al. (2012)Methods Enzymol.,512, 107.

Linkage

Replaces: 04-12-1263

Analysis Note

Color (visual): white to light yellow to beige
Appearance of substance (visual): powder
Identity (IR): passes test
Optical rotation α 25/D (c=1 in DMF): -13.0 - -8.5 °
Purity (TLC(CMA2)): ≥ 98 %
Assay (HPLC, area%): ≥ 97.0 % (a/a)
Solubility (1 mmole in 2 mL DMF): clearly soluble

To see the solvent systems used for TLC of Novabiochem^®products please click here.Novabiochem is a registered trademark of Merck KGaA, Darmstadt, Germany