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GenScript Gencrispr T7 Endonuclease I; 1250U (10000 U/mL)
List Price $220.00 Your Price $220.00
GenScript Gencrispr T7 Endonuclease I - GSCRPT (Additional S&H or Hazmat Fees May Apply)
NETA PART: GSCRPT-Z03396-1250
MFG.PART: Z03396-1250
UNSPSC: 12352204
Manufacturer: GenScript USA Inc
Description
T7 Endonuclease I (T7E1) recognizes and cleaves non-perfectly matched DNA, cruciform DNA structures, Holliday structures or junctions, hetero duplex DNA and more slowly, nicked double-stranded DNA. The cleavage site is at the first, second or third phosphodiester bond that is 5´ to the mismatch. The protein is the product of T7 gene 3. GenCrispr T7 Endonuclease I is a fusion protein produced from E.coli. |
Synonyms
T7E1
Note
T7 Endonuclease I is a structure-selective enzyme. It acts on a variety of DNA substrates with different specific activities. It is important to control the amount of enzyme and the reaction time used for cleavage of a particular substrate. Temperatures above 42°C cause an increase in nonspecific nuclease activity and should be avoided.
Applications
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Properties
| Storage & Stability | GenCrispr T7 Endonuclease I is supplied in 1X storage buffer (200 mM NaCl,20 mM Tris-HCl（pH 7.5）, 0.1 mM EDTA, 1 mM dithiothreitol, 0.15% Triton X-100 and 50% glycerol). The recommended storage temperature is -20°C. |
| SKU | GSCRPT-Z03396-1250 |
|---|---|
| Supplier Part Number | Z03396-1250 |
| UM | EA |
| UNSPSC | 12352204 |
| Manufacturer | GenScript USA Inc |
| Temperature | GenCrispr T7 Endonuclease I is supplied in 1X storage buffer (200 mM NaCl,20 mM Tris-HCl（pH 7.5）, 0.1 mM EDTA, 1 mM dithiothreitol, 0.15% Triton X-100 and 50% glycerol). The recommended storage temperature is -20°C. |
| ProductLine | GSCRPT |
| Qty | 1 |
| MinOrderQty | 1 |
| Weight | 7.000000 |
| Lead Time | 7 |
| Hazardous | N |
| Energy Star | No |
| Green | No |
| Controlled | N |