We use cookies to make your experience better. To comply with the new e-Privacy directive, we need to ask for your consent to set the cookies. Learn about our cookie policy.
GenScript Gencrispr™ Sacas9 2nls Nuclease; 0.1mg
List Price $188.00 Your Price $188.00
GenScript Gencrispr™ Sacas9 2nls Nuclease0.1mg - GSCRPT (Additional S&H or Hazmat Fees May Apply)
NETA PART: GSCRPT-Z03699-0.1
MFG.PART: Z03699-0.1
UNSPSC: 12352204
Manufacturer: GenScript USA Inc
Description
The GenCRISPR™SaCas9 2NLS Nuclease can be formed with the guide RNA into a ribonucleoprotien(RNP) complex. The use of an RNP complex to perform gene editing has been shownto reduce the challenges encountered with other CRISPR gene editing techniquessuch as viral and plasmid delivery. Challenges include off-target effects, cellviability and transcription/translational challenges. The SaCas9 recognizes anNNGRRT protospacer adjacent motif (PAM) and cleaves target DNA at highefficiency with a variety of guide RNA (gRNA) spacer lengths. GenCRISPR™ SaCas9 2NLSNuclease is a tag free nuclease produced by expression in an E. coli strain carrying a plasmidencoding the Cas9 gene from Staphylococcusaureus with a nuclear localization signal at both N-terminal and C-terminal.Thesmall size of the nuclease facilitates enhanced in vivo delivery for genomeediting in various organisms. |
Note
For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.
Applications
| gRNA-dependent double-stranded DNA cleavage |
Properties
| Expression System | RecombinantSaCas9 with an NLS at both N-terminal and C-terminal expressed by E.coli |
| Species | Staphylococcus aureus |
| Tag | Tag-free |
| Molecular Weight | ~130kDa |
| Concentration | 4 mg/ml |
| Active temperature | ThisSaCas9 is active at 37°C. |
| Formulation | Supplied as a solution of 20 mM Tris, 300 mM NaCl, 0.1mM TCEP, 50% glycerol, pH 7.5 |
| Storage & Stability | This product remains stable up to 12 months at -20°C. Avoid repeated freeze-thaw cycles |
| Key Features | High knockoutefficiencies: Consistent high performance in in-vitro plasmid cleavage test. Tag-free: No extra tagamino acid. DNA-free: Noexternal DNA added to the system. |
Quality Control Specifications
| Appearance | Clear,colorless liquid |
| Purity | ≥ 90%as analyzed by SDS-PAGE |
| Concentration by A280 | 4mg/ml±10% |
| Bioactivity (in vitro) | ≥ 90% |
| Residual DNase | Non-specificDNase activity |
| Residual RNase | Non-specificRNase activity |
| Endotoxin Level | ≤100 EU/mg as analyzed by gel clotting method |
| SKU | GSCRPT-Z03699-0.1 |
|---|---|
| Supplier Part Number | Z03699-0.1 |
| UM | EA |
| UNSPSC | 12352204 |
| Manufacturer | GenScript USA Inc |
| Temperature | This product remains stable up to 12 months at -20°C. Avoid repeated freeze-thaw cycles |
| ProductLine | GSCRPT |
| Qty | 1 |
| MinOrderQty | 1 |
| Weight | 7.000000 |
| Lead Time | 7 |
| Hazardous | N |
| Energy Star | No |
| Green | No |
| Controlled | N |