Takara Lenti-X GoStix Plus \50 Tests

Lenti-X GoStix Plus provide a rapid, simple, and effective method for instantly quantifying lentivirus in packaging cell supernatants. The test involves applying 20 µl of supernatant to a GoStix cassette and waiting 10 minutes for the appearance of test and control bands that indicate the presence of lentiviral p24. The results on the cassette can then be analyzed using a free smartphone app, which quantifies lentivirus titer by comparing the intensities of the test and control bands.

The quantitative output from the Lenti-X GoStix App (referred to as a GoStix Value, or GV) can be used to compare virus amounts between different preparations in a manner similar to, but far easier and quicker than, an ELISA, and allows for calculation of actual IFU/mL using a reference virus.

Cat. # 631281 includes sufficient reagents and cassettes for performing 50 tests.

p24 production, GoStix Value, and IFU/mL are dependent on harvest time. ZsGreen1 lentivirus preparations were made in duplicate in accordance with the manufacturers instructions using Lenti-X Single Shots and harvested at the times indicated. At each timepoint, samples were analyzed for GV using Lenti-X GoStix Plus and virus dilutions were titrated on HT1080 cells to determine IFU/mL. In addition to showing that GV (i.e., p24 accumulation) and IFU/mL are dependent on harvest time, these data demonstrate that the ratio between GV and IFU/mL changes over time.