Takara BacPAK Baculovirus Expression System \Each

Complete kit for expressing recombinant proteins at high levels. The BacPAK System uses the baculovirusAutographa californicanuclear polyhedrosis virus (AcMNPV) to produce target proteins in insect cells. More than 90% of the viruses produced by the transfected cells carry the target protein. The expressed recombinant protein is similar in structure, biological activity, and immunological reactivity to the naturally occurring protein because insect host cells provide post-translational processing similar to that of mammalian cells.

Required Products

IPLB-Sf21 insect cells may be used as a host for propagating theAutographa californicamultiple-enveloped nuclear polyhedrosis virus (AcMNPV) and its expression vector derivatives generated from ourBacPAKsystem. The IPLB-Sf21 cell line isderived from pupal ovaries of the fall armyworm,Spodoptera frugiperda. Exponentially growing IPLB-Sf21 cells are concentrated by centrifugation and frozen in insect cell complete medium containing 10% dimethysulfoxide (DMSO).