Takara Retro-X Universal Packaging System \Each

This kit provides a complete retroviral vector packaging system for use with any retroviral vector. It is designed to produce virus that can infect dividing cells from a broad range of mammalian and nonmammalian cell types. The GP2-293 Packaging Cell Line contains only the MoMuLVgagandpolgenes. The viral envelope portion of the packaging function (envgene) is supplied by transiently co-transfecting one of the Retro-X Universal Packaging Vectors (p10A1, pAmpho, pEco, or pVSV-g) with the retroviral vector. The pQCLIN Retroviral Vector (included in the vector set) can be used as a positive control to constitutively express β-galactosidase in transduced mammalian cells.

High titer retroviral packaging with a choice of four different retroviral packaging envelopes.GP2-293 Cells were transfected with a Retro-X Q vector expressing the red fluorescent protein DsRed2, and each of the four envelope vectors supplied with the Retro-X Unversal Packaging System. After incubating the transfected GP2-293 cells for 48 hr, 10 ?L of the resulting virus was used to infect NIH 3T3 cells. After 48 hours, titers were measured by flow cytometry. Titers were calculated by multiplying the percent infected cells (infectious units) by the total cell number, then dividing by the volume of virus (mL).Panel A. Ecotropic envelope.Panel B. Amphotropic envelope.Panel C. Dualtropic envelope.Panel D. VSV-g envelope (pantropic). Positive cells are indicated by the red line. Negative control cells are indicated by the green line.