Takara iDimerize Regulated Transcription System \Each

The iDimerize Regulated Transcription System allows tightly regulated, inducible expression of your gene of interest by the addition of a small molecule to the cell medium. The system includes the iDimerize Regulated Transcription Vector Set 2, which contains a vector that encodes two transcription factor domains (which combine to create the inducible transcription factor), a mammalian expression vector used to express your gene of interest, and a control vector. The system also includes the A/C Heterodimerizer, the membrane-permeant compound required for induction of gene expression through the heterodimerization of the two transcription factor domains.

Tightly regulated transcription using iDimerize technology.Clone your gene of interest downstream of theZHFD1inducible promoter (PZI-1). The DNA binding component (DmrC/DNA-BD fusion; red) recognizes and binds sequences within the promoter. However, activation of transcription only occurs when the DmrC/DNA-BD dimerizes with the transcription activation component (DmrA-AD fusion; green) at the promoter, when the DmrA and DmrC domains both bind to the A/C Heterodimerizer (AP21967).