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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M | IP, WB | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | 04-002 |
| Brand Family | Upstate |
| Trade Name |
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| Description | Anti-SOCS1 Antibody, clone 4H1 |
| Alternate Names |
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| Product Information | |
|---|---|
| Format | Purified |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-SOCS1 Antibody, clone 4H1 is an antibody against SOCS1 for use in IP & WB. |
| Key Applications |
|
| Application Notes | Immunoblot Analysis: 1:1,000 dilution of this antibody detected SOCS1 in KG-1 cell lysate. Immunoprecipitation (IP): 10 μg of this antibody immunoprecipitated SOCS1 from 200 μL of cell extract in RIPA buffer. |
| Biological Information | |
|---|---|
| Immunogen | mouse SOCS1 recombinant GST fusion protein encompassing amino acids 2-78. |
| Clone | 4H1 |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Mouse |
| Specificity | Recognizes SOCS1 |
| Isotype | IgG1 |
| Species Reactivity |
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| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Entrez Gene Summary | This gene encodes a member of the STAT-induced STAT inhibitor (SSI), also known as suppressor of cytokine signaling (SOCS), family. SSI family members are cytokine-inducible negative regulators of cytokine signaling. The expression of this gene can be induced by a subset of cytokines, including IL2, IL3 erythropoietin (EPO), CSF2/GM-CSF, and interferon (IFN)-gamma. The protein encoded by this gene functions downstream of cytokine receptors, and takes part in a negative feedback loop to attenuate cytokine signaling. Knockout studies in mice suggested the role of this gene as a modulator of IFN-gamma action, which is required for normal postnatal growth and survival. |
| Gene Symbol |
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| Purification Method | Protein G Purified |
| UniProt Number |
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| UniProt Summary | FUNCTION: SwissProt: O15524 # SOCS family proteins form part of a classical negative feedback system that regulates cytokine signal transduction. SOCS1 is involved in negative regulation of cytokines that signal through the JAK/STAT3 pathway. Through binding to JAKs, inhibits their kinase activity. In vitro, also suppresses Tec protein- tyrosine activity. Appears to be a major regulator of signaling by interleukin 6 (IL6) and leukemia inhibitory factor (LIF). Regulates interferon-gamma mediated sensory neuron survival (By similarity). Probable substrate-recognition component of a SCF- like ECS (Elongin-Cullin-SOCS-box protein) E3 ubiquitin ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Seems to recognize JAK2. SIZE: 211 amino acids; 23551 Da SUBUNIT: Interacts with multiple activated signaling proteins of the tyrosine kinase signaling pathway including JAK family kinases, TEC, KIT, GRB2 and VAV. Binding to JAKs is mediated through the KIR and SH2 domains to a phosphorylated tyrosine residue within the JAK JH1 domain. Binds the SH3 domain of GRB2 via diproline determinants in the N-terminus, and the N-terminal regulatory domain of VAV (By similarity). Interacts with the Elongin BC complex (TCEB1 and TCEB2). Component of an ECS CBC(VHL) E3 ubiquitin ligase complex which contains Elongin BC complex, CUL5, RBX1 and SOCS1 (By similarity). Interacts with TRIM8 (By similarity). Interacts with CUL2. TISSUE SPECIFICITY: Expressed in all tissues with high expression in spleen, small intestine and peripheral blood leukocytes. DOMAIN: SwissProt: O15524 The ESS and SH2 domains are required for JAK phosphotyrosine binding. Further interaction with the KIR domain is necessary for signal and kinase inhibition. & The SOCS box domain mediates the interaction with the Elongin BC complex, an adapter module in different E3 ubiquitin ligase complexes. The Elongin BC complex binding domain is also known as BC-box with the consensus [APST]-L-x(3)-C-x(3)-[AILV] and is part of the SOCS box (By similarity). SIMILARITY: Contains 1 SH2 domain. & Contains 1 SOCS box domain. |
| Molecular Weight | 37 kDa |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Routinely evaluated by immunoblot. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | 2 years at 2° to 8°C from date of shipment. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |