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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | WB | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | 04-135 |
| Brand Family | Upstate |
| Trade Name |
|
| Description | Anti-Brachyury Antibody, clone 3E4.2 |
| Alternate Names |
|
| Background Information | The Brachyury protein is a T-box transcription factor that is one of the earliest indicators of mesoderm formation during embryonic development. As the brachyury protein family is essential for the formation of the posterior body in all vertebrates, Brachyury has been shown to be expressed during gastrula and neurula stages, as well as in the developing notochord. Consequently, when mutations occur within the brachyury gene, severe vertebral malformations have been found to occur, as a direct result. In addition, current findings also indicate that the dysregulation of brachyury may also be linked to the formation of chordomas, which are malignant tumors that develop along the spine. |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified mouse monoclonal IgG1κ in buffer containing PBS with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This Anti-Brachyury Antibody, clone 3E4.2 is validated for use in WB for the detection of Brachyury. |
| Key Applications |
|
| Biological Information | |
|---|---|
| Immunogen | Synthetic human brachyury peptide, a.a. 224-435. |
| Epitope | C-terminus |
| Clone | 3E4.2 |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Mouse |
| Specificity | This monoclonal antibody recognizes the brachyury protein. |
| Isotype | IgG1κ |
| Species Reactivity |
|
| Species Reactivity Note | Reacts with human. Reactivity with other species has not been tested. |
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
|
| Entrez Gene Summary | The protein encoded by this gene is an embryonic nuclear transcription factor that binds to a specific DNA element, the palindromic T-site. It binds through a region in its N-terminus, called the T-box, and effects transcription of genes required for mesoderm formation and differentiation. The protein is localized to notochord-derived cells. |
| Gene Symbol |
|
| Purification Method | Protein G Purified |
| UniProt Number |
|
| UniProt Summary | FUNCTION: SwissProt: O15178 # Involved in the transcriptional regulation of genes required for mesoderm formation and differentiation. Binds to a palindromic site (called T site) and activates gene transcription when bound to such a site. SIZE: 435 amino acids; 47443 Da SUBUNIT: Monomer (By similarity). Nucleus. DISEASE: SwissProt: O15178 # Genetic variations in T are associated with susceptibility to neural tube defects (NTD) [MIM:182940]. NTD are common congenital malformations. Spina bifida, which results from malformations in the caudal region of the neural tube, is compatible with life but associated with significant morbidity, including lower limb paralysis. SIMILARITY: SwissProt: O15178 ## Contains 1 T-box DNA-binding domain. |
| Stem Cell Type |
|
| Molecular Weight | 47 kDa |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Routinely evaluated by Western blot on NT2 cell lysates. Western Blotting: Recommended working dilutions are 1:1000. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Maintain refrigerated at 2-8°C in undiluted aliquots for up to 12 months. Avoid repeat freeze/thaw cycles. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |