Special Offers
100% Performance Guaranteed
Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | IHC, WB, IP | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | 04-145 |
| Description | Anti-NOD2 Antibody, clone 2D9 |
| Alternate Names |
|
| Background Information | NOD2 (nucleotide-binding oligomerization domain containing 2), also known as the caspase recruitment domain family, member 15 (CARD15), is a member of the NOD-like Receptor (NLR) family of proteins and plays an important role in the immune system. It is an intracellular pattern recognition receptor and is linked to inflammatory diseases such as inflammatory bowel disease/Crohns Disease and Blau syndrome. NOD2 has two caspase recruitment domains (CARDs) and eleven leucine-rich repeats (LRRs). The protein is primarily expressed in the peripheral blood leukocytes. It plays a role in the immune response by recognizing the bacterial molecules. Recently it is has been found to interact with NLRC4 and bind to MAVS in response to ssRNA or viral RNA treatment and activate the IFN response. |
| Product Information | |
|---|---|
| Format | Purified |
| Control |
|
| Presentation | Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-NOD2 Antibody, clone 2D9 is a Mouse Monoclonal Antibody for detection of NOD2 also known as NOD-like receptor C2 & has been validated in IHC, WB & IP. |
| Key Applications |
|
| Biological Information | |
|---|---|
| Immunogen | His-tagged recombinant protein corresponding to human NOD2. |
| Epitope | Unknown |
| Clone | 2D9 |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Mouse |
| Specificity | This antibody recognizes NOD2. |
| Isotype | IgG1κ |
| Species Reactivity |
|
| Species Reactivity Note | Proven to react with human. |
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
|
| Entrez Gene Summary | This gene is a member of the Nod1/Apaf-1 family and encodes a protein with two caspase recruitment (CARD) domains and six leucine-rich repeats (LRRs). The protein is primarily expressed in the peripheral blood leukocytes. It plays a role in the immune response to intracellular bacterial lipopolysaccharides (LPS) by recognizing the muramyl dipeptide (MDP) derived from them and activating the NFKB protein. Mutations in this gene have been associated with Crohns disease and Blau syndrome. [provided by RefSeq]. |
| Gene Symbol |
|
| Purification Method | Protein G Purified |
| UniProt Number |
|
| UniProt Summary | FUNCTION: Induces NF-kappa-B via RICK (CARDIAK, RIP2) and IKK-gamma. Confers responsiveness to intracellular bacterial lipopolysaccharides (LPS). SUBUNIT STRUCTURE: Binds to RICK by CARD-CARD interaction. SUBCELLULAR LOCATION: Cytoplasm. TISSUE SPECIFICITY: Monocytes-specific. INVOLVEMENT IN DISEASE: Defects in NOD2 are the cause of Blau syndrome (BS) [MIM:186580]. BS is a rare autosomal dominant disorder characterized by early-onset granulomatous arthritis, uveitis and skin rash. Defects in NOD2 are a cause of susceptibility to Crohns disease (CD) [MIM:266600]. CD is a form of remitting inflammatory bowel disease (IBD). CD may involve any part of the gastrointestinal tract, but most frequently the terminal ileum and colon. Bowel inflammation is transmural and discontinuous. Crohns disease is commonly classified as autoimmune disease. Defects in NOD2 are a cause of susceptibility to ulcerative colitis [MIM:191390]. It is a chronic inflammatory bowel disease. In ulcerative colitis, the inflammation is continuous and limited to rectal and colonic mucosal layers. Ulcerative colitis is commonly classified as autoimmune disease. Defects in NOD2 are the cause of early-onset sarcoidosis (EOS) [MIM:609464]. Sarcoidosis is a multiorganic inflammatory disease with unknown etiology, characterized by the histologic features of non-caseating epithelioid granulomas. In childhood, 2 distinct types of sarcoidosis have been described. Usually the disease is detected in older children by chest radiography and the clinical manifestations are characterized by a classical triad of lung, lymph node, and eye involvement, similar to those in adults. In contrast, early-onset sarcoidosis (EOS), which usually appears in those younger than 4 years of age, is quite rare and has a distinct triad of skin, joint, and eye disorders, without apparent pulmonary involvement. Compared with an asymptomatic and sometimes naturally disappearing course of the disease in older children, EOS is progressive and in many cases causes severe complications, such as blindness, joint destruction, and visceral involvement. SEQUENCE SIMLARITIES: Contains 2 CARD domains. Contains 6 LRR (leucine-rich) repeats. Contains 1 NACHT domain. |
| Molecular Weight | ~110 kDa |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Immunohistochemistry Analysis: A 1:25 dilution of this antibody detected NOD2 in hyperplastic colorectal carcinoma tissue. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |