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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | WB, IP, IHC, ChIP | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | 04-1506 |
| Replaces | MABE547 |
| Description | Anti-CTCF Antibody, clone 1.2.1.5.4 |
| Alternate Names |
|
| Background Information | CTCF, also known as CCCTC-binding factor, contains 11 zinc finger (ZF) binding domains that allow it to bind different DNA target sequences and proteins. This multifunctional protein can act as a transcriptional activator or repressor. As a transcriptional repressor, CTCF, binds to promoters of vertebrate c-myc gene. It also binds to the PLK and PIM1 promoters. CTCF may prevent the access of transcriptional activators to enhancers. CTCF also acts as a transcriptional activator of APP. It is involved in different aspects of gene regulation including promoter activation or repression, hormone-responsive gene silencing, methylation-dependent chromatin insulation, and genomic imprinting. Mutations of CTCF in this gene have been associated with invasive breast cancers, prostate cancers, and Wilms tumors, suggesting a role for CTCF may as a tumor suppressor. |
| Product Information | |
|---|---|
| Format | Purified |
| Control |
|
| Presentation | Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mm NaCl) with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-CTCF Antibody, clone 1.2.1.5.4 is a Mouse Monoclonal Antibody for detection of CTCF also known as 11-zinc finger protein, CCCTC-binding factor & has been validated in WB, IP & IHC. |
| Key Applications |
|
| Application Notes | Chromatin Immunoprecipitation Analysis: A representative lot was used by an independent laboratory in ChIP. (Pugacheva, E., et Al. (2005). Human Molecular Genetics. 14(7):953–965.) |
| Biological Information | |
|---|---|
| Immunogen | His-tagged recombinant protein corresponding to human CTCF. |
| Epitope | Unknown |
| Clone | 1.2.1.5.4 |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Mouse |
| Specificity | This antibody recognizes CTCF. |
| Isotype | IgG1κ |
| Species Reactivity |
|
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
|
| Entrez Gene Summary | This gene is a member of the BORIS + CTCF gene family and encodes a transcriptional regulator protein with 11 highly conserved zinc finger (ZF) domains. This nuclear protein is able to use different combinations of the ZF domains to bind different DNA target sequences and proteins. Depending upon the context of the site, the protein can bind a histone acetyltransferase (HAT)-containing complex and function as a transcriptional activator or bind a histone deacetylase (HDAC)-containing complex and function as a transcriptional repressor. If the protein is bound to a transcriptional insulator element, it can block communication between enhancers and upstream promoters, thereby regulating imprinted expression. Mutations in this gene have been associated with invasive breast cancers, prostate cancers, and Wilms tumors. [provided by RefSeq]. |
| Gene Symbol |
|
| Purification Method | Protein g Purified |
| UniProt Number |
|
| UniProt Summary | FUNCTION: Transcriptional repressor binding to promoters of vertebrate c-myc gene. Also binds to the PLK and PIM1 promoters. May prevent the access of transcriptional activators to enhancers. Also acts as a transcriptional activator of APP. Involved in different aspects of gene regulation including promoter activation or repression, hormone-responsive gene silencing, methylation- dependent chromatin insulation, and genomic imprinting. Seems to act as tumor suppressor.r/>SIZE: 727 amino acids; 82785 Da SUBCELLULAR LOCATION: Nucleus. TISSUE SPECIFICITY:Ubiquitous. Absent in primary spermatocytes. SIMILARITY: Belongs to the CTCF zinc-finger protein family. & Contains 11 C2H2-type zinc fingers. |
| Molecular Weight | ~ 130 kDa Note: Poly(ADP-ribosyl)ated isoform, C-terminal truncation, or sumoylation will cause variable migration of CTCF protein on SDS–PAGE, which may present CTCF with different molecular weight on Western Blot. Modifications are dependent on cell type and growing condition. The following molecular weights have been reported in the literature: 180 kDa (CTCF-180), 130 kDa (CTCF-130), 82 kDa (CTCF-82), 70 kDa (CTCF-70). See Klenova,E.M.,et Al, (1997) Nucleic Acids Res. 25, 466–473 and Torrano,V,et Al, (2006) Journal of Cell Science 119, 1746-1759) for additional details. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blot in K562 cell lysate. Western Blot Analysis: 1 µg/mL of this antibody detected CTCF on 10 µg of K562 cell lysate. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |