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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | WB, IP, ICC, IHC, FC, ChIP | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | 04-1555 |
| Description | Anti-Mdmx Antibody, clone 8C6 |
| Alternate Names |
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| Background Information | Mdmx is a Mdm2-related protein that like Mdm2 has been shown to bind to and inhibit p53, although not as well as Mdm2. This interaction with p53 occurs via a binding domain located in the N-terminal region of the protein. Mdmx has been shown to counteract Mdm2-dependent p53 degradation and to stabilize p53 in its inactive form. Although Mdmx has significant structural similarity to Mdm2 E3 ubiquitin ligase, most studies have not been able to ascribe E3 ligase function to Mdmx. |
| Product Information | |
|---|---|
| Format | Purified |
| Control |
|
| Presentation | Purified mouse monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mm NaCl) with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-Mdmx Antibody, clone 8C6 is a Mouse Monoclonal Antibody for detection of Mdmx also known as MDM4-related protein 1, Mdm2-like p53-binding protein & has been validated in WB, IP, ICC, IHC, FC. |
| Key Applications |
|
| Application Notes | Chromatin Immunoprecipitation Analysis: A representative lot was used by an independent laboratory for this application. (Gilkes, D., et Al. (2008). Molecular and Cellular Biology. 28(6):1999-2010.) Immunohistochemistry Analysis: A representative lot was used by an independent laboratory for this application. (Gilkes, D., et Al. (2008). Molecular and Cellular Biology. 28(6):1999-2010.) Immunoprecipitation Analysis: A representative lot was used by an independent laboratoryfor this application. (Changgong, L., et Al. (2002). Molecular and Cellular Biology. 22(21):7562-7571.) Flow Cytometry Analysis: A previous lot was used by an independent laboratory for this application. (Changgong, L., et Al. (2002). Molecular and Cellular Biology. 22(21):7562-7571.) |
| Biological Information | |
|---|---|
| Immunogen | His-tagged recombinant protein corresponding to human Mdmx. |
| Epitope | Unknown |
| Clone | 8C6 |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Mouse |
| Specificity | This antibody recognizes Mdmx. |
| Isotype | IgG2bκ |
| Species Reactivity |
|
| Species Reactivity Note | Proven to react with human. |
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
|
| Entrez Gene Summary | The human MDM4 gene(encodes Mdmx protein), which plays a role in apoptosis, encodes a 490-amino acid protein containing a RING finger domain and a putative nuclear localization signal. The MDM4 putative nuclear localization signal, which all Mdm proteins contain, is located in the C-terminal region of the protein. The mRNA is expressed at a high level in thymus and at lower levels in all other tissues tested. MDM4 protein produced by in vitro translation interacts with p53 via a binding domain located in the N-terminal region of the MDM4 protein. MDM4 shows significant structural similarity to p53-binding protein MDM2. Two transcript variants, one protein-coding and the other likely not to be protein-coding, have been found for this gene. [provided by RefSeq] |
| Gene Symbol |
|
| Purification Method | Protein g Purified |
| UniProt Number |
|
| UniProt Summary | FUNCTION: Inhibits p53- and p73-mediated cell cycle arrest and apoptosis by binding its transcriptional activation domain. Inhibits degradation of MDM2. Can reverse MDM2-targeted degradation of p53 while maintaining suppression of p53 transactivation and apoptotic functions. SUBUNIT STRUCTURE: Binds to p53, p73 and MDM2. SUBCELLULAR LOCATION: Nucleus. TISSUE SPECIFICITY: In all tissues tested, with high levels in thymus. DOMAIN: Region I is sufficient for binding p53 and inhibiting its G1 arrest and apoptosis functions. It also binds p73. Region II contains most of a central acidic region and a putative C4-type zinc finger. The RING finger domain which coordinates two molecules of zinc mediates the heterooligomerization with MDM2. SEQUENCE SIMILARITIES: Belongs to the MDM2/MDM4 family. Contains 1 RanBP2-type zinc finger. Contains 1 RING-type zinc finger. Contains 1 SWIB domain. MASS SPECTROMETRY: Molecular mass is 54863.3 Da from positions 1 - 490. Determined by MALDI. |
| Molecular Weight | ~ 80 kDa |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blot in MCF-7 cell lysate. Western Blot Analysis: 0.5 µg/mL of this antibody detected Mdmx on 10 µg of MCF-7 cell lysate. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |