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Millipore 100ul, Monoclonal, Rat, Culture Supernatant, Liquid
Millipore 100ul, Monoclonal, Rat, Culture Supernatant, Liquid
List Price$561.66Your Price$561.66
Millipore Anti-Rna Polymerase Ii Subunit B1 (Phospho Ctd Ser-2), Clone 3e1 (Rat Monoclonal) Is A Highly Specific Rat Monoclonal Antibody & That Targets Rna Polymerase & Has Been Tested In Western Blott - Mill (Additional S&H or Hazmat Fees May Apply)
RPB1 (RNA polymerase II subunit B1) is the catalytic component of RNA polymerase II which synthesizes mRNA and non-coding RNAs. During transcription, elongation is influenced by the phosphorylation status of the C-terminal domain (CTD) of RPB1, which acts as an assembly platform for factors regulating transcription initiation, elongation, termination and mRNA processing. Phosphorylation occurs mainly at residues Ser-2 and Ser-5 of the tandem 7 residue repeats in the C-terminal domain (CTD), which activates Pol II. This phosphorylation also can occur at Ser-7 of the heptapepdtide repeat. This antibody recognizes the Ser-2 CTD residue of RPB1.
Product Information
Format
Culture Supernatant
Control
Untreated and lambda phosphatase treated NIH/3T3 cell lysate
Presentation
Unpurified rat monoclonal IgG1κ tissue culture supenatant with 0.05% sodium azide.
Quality Level
MQ100
Applications
Application
Anti-RNA polymerase II subunit B1 (phospho CTD Ser-2), clone 3E1 (rat monoclonal) is a highly specific rat monoclonal antibody & that targets RNA Polymerase & has been tested in western blotting.
Key Applications
Chromatin Immunoprecipitation (ChIP)
Western Blotting
ELISA
Application Notes
Chromatin Immunoprecipitation Analysis: A representative lot was used by an independent laboratory to immunoprecipitate RNA polymerase II subunit B1 (phospho-CTD Ser-5) in ChIP (Chapman, R., et Al. (2007). Science. 318(5857):1780 -1782.). Demonstrated to react with human RBP1 in Ni et Al., 2011. Transcription 2:5, 237-242
Biological Information
Immunogen
Ovalbumin-conjugated linear peptide corresponding to human RNA Polymerase II subunit B1 phosphorylated at Ser2 of the C-terminal domain (CTD).
Epitope
Phosphorylated Ser2 at the C-terminal domain (CTD)
Clone
3E10
Host
Rat
Specificity
This antibody recognizes RNA Polymerase II subunit B1 phosphorylated at Ser2 of the C-terminal domain (CTD).
Isotype
IgG1κ
Species Reactivity
Mouse
Human
Species Reactivity Note
Demonstrated to react with Mouse. Predicted to react with Human based upon immunogen design. Demonstrated to react with human RBP1 in Ni et Al., 2011. Transcription 2:5, 237-242
Antibody Type
Monoclonal Antibody
Entrez Gene Number
NP_000928
Gene Symbol
POLR2A
POLR2
Purification Method
Unpurified
UniProt Number
P24928
Molecular Weight
~220 kDa observed
Product Usage Statements
Quality Assurance
Evaluated by Western Blotting in untreated and lambda phosphatase treated NIH/3T3 cell lysate.
Western Blotting Analysis: A 1:2,000 dilution of this antibody detected RNA Polymerase II subunit B1 (phospho CTD Ser-2) in untreated NIH/3T3 cell lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
