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Millipore Anti-Rna Polymerase Ii Subunit B1 (Phospho-Ctd Ser-5) Antibody
Millipore Anti-Rna Polymerase Ii Subunit B1 (Phospho-Ctd Ser-5) Antibody
List Price$590.52Your Price$590.52
Millipore Anti-Rna Polymerase Ii Subunit B1 (Phospho-Ctd Ser-5) Antibody, Clone 3e8 Is A Highly Specific Rat Monoclonal Antibody, That Targets Rna Polymerase & Has Been Tested In Western Blotting & Elis - Mill (Additional S&H or Hazmat Fees May Apply)
Anti-RNA polymerase II subunit B1 (phospho-CTD Ser-5) Antibody, clone 3E8
Alternate Names
DNA-directed RNA polymerase II subunit RPB1
RNA polymerase II subunit B1
DNA-directed RNA polymerase II subunit A
DNA-directed RNA polymerase III largest subunit
RNA-directed RNA polymerase II subunit RPB1
Background Information
RPB1 (RNA polymerase II subunit B1) is the catalytic component of RNA polymerase II which synthesizes mRNA and non-coding RNAs. During transcription, elongation is influenced by the phosphorylation status of the C-terminal domain (CTD) of RPB1, which acts as an assembly platform for factors regulating transcription initiation, elongation, termination and mRNA processing. Phosphorylation occurs mainly at residues Ser-2 and Ser-5 of the tandem 7 residue repeats in the C-terminal domain (CTD), which activates Pol II. This phosphorylation also can occur at Ser-7 of the heptapepdtide repeat. This antibody recognizes the Ser-5 CTD residue of RPB1.
Product Information
Format
Culture Supernatant
Control
Untreated and lambda phosphotase treated NIH/3T3 cell lysates
Presentation
Rat monoclonal IgG2aκ with 0.05% sodium azide.
Quality Level
MQ100
Applications
Application
Anti-RNA polymerase II subunit B1 (phospho-CTD Ser-5) Antibody, clone 3E8 is a highly specific rat monoclonal antibody, that targets RNA Polymerase & has been tested in western blotting & ELISA.
Key Applications
Chromatin Immunoprecipitation (ChIP)
Western Blotting
ELISA
Application Notes
Chromatin Immunoprecipitation Analysis: A representative lot was used by an independent laboratory to immunoprecipitate RNA polymerase II subunit B1 (phospho-CTD Ser-5) in ChIP (Chapman, R., et Al. (2007). Science. 318(5857):1780 -1782.).
ELISA Analysis: A representative lot was used by an independent laboratory to detect RNA polymerase II subunit B1 (phospho-CTD Ser-5) in ChIP (Chapman, R., et Al. (2007). Science. 318(5857):1780 -1782.).
Biological Information
Immunogen
Ovalbumin-conjugated linear peptide corresponding to human RNA Polymerase II subunit B1 phosphorylated at Ser5 of the C-terminal domain (CTD).
Epitope
Phosphorylated Ser5 at the C-terminus domain (CTD).
Clone
3E8
Host
Rat
Specificity
This antibody recognizes RNA Polymerase II subunit B1 phosphorylated at Ser5 of the C-terminus domain (CTD).
Isotype
IgG2aκ
Species Reactivity
Mouse
Species Reactivity Note
Demonstrated to react with Mouse. Predicted to react with Human based upon immunogen design.
Antibody Type
Monoclonal Antibody
Entrez Gene Number
NP_000928
Gene Symbol
POLR2A
POLR2
Purification Method
Unpurified
UniProt Number
P24928
Molecular Weight
~220 kDa observed
Product Usage Statements
Quality Assurance
Evaluated by Western Blot in untreated and lambda phosphotase treated NIH/3T3 cell lysates.
Western Blot Analysis: A 1:2,000 dilution from a representative lot detected RNA polymerase II subunit B1 (phospho-CTD Ser-5) in 10 µg of untreated and lambda phosphotase treated NIH/3T3 cell lysates.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
