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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| R, M, H | WB | M | Ascites | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | 04-1573 |
| Description | Anti-Ataxin-7 Antibody, clone 3SCA-1C1 |
| Alternate Names |
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| Background Information | Ataxin-7 is a protein of unknown function. However, since this protein contains a nuclear localization sequence and is found to be localized in the nucleus, it has been postulated to be a potential transcription factor. Spinocerebellar ataxia-7 (one of a group of hereditary neurodegenrative diseases) is caused by an expanded CAG repeat in the gene encoding ataxin-7 (the diseased allele associated with Spinocerebellar ataxia-7, contains 38-130 CAG repeats (near the N-terminus), compared to 7-17 in the normal allele). In cells where there is a mutation of the SCA7 gene, ataxin-7 accumulates in intranuclear inclusions and can result in cell death. Spinocerebellar ataxia (SCA) is one of a group of genetic disorders characterized by slowly progressive incoordination of gait and often associated with poor coordination of hands, speech, and eye movements. Frequently, atrophy of the cerebellum occurs. |
| Product Information | |
|---|---|
| Format | Ascites |
| Control |
|
| Presentation | Unpurified mouse IgG1, k antibody in ascites fluid without preservatives. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-Ataxin-7 Antibody, clone 3SCA-1C1 is an antibody against Ataxin-7 for use in WB. |
| Key Applications |
|
| Application Notes | ELISA: A 1:500-1:5,000 dilution of a previous lot of 04-1573 was used in ELISA, as reported by an outside laboratory. Immunocytochemistry: A 1:500-1:5,000 dilution of a previous lot of 04-1573 was used in IC, as reported by an outside laboratory. |
| Biological Information | |
|---|---|
| Immunogen | Recombinant human Ataxin 7 as a fusion protein (aa 1 to aa 229). |
| Epitope | Epitope mapped between aa 66 and aa 119. |
| Clone | clone 3SCA-1C1 |
| Host | Mouse |
| Specificity | This antibody recognizes Ataxin-7 protein. |
| Isotype | IgG1κ |
| Species Reactivity |
|
| Species Reactivity Note | Demonstrated to react with Rat and Mouse. Predicted to react with Human based on immunogen design. |
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Entrez Gene Summary | The autosomal dominant cerebellar ataxias (ADCA) are a heterogeneous group of neurodegenerative disorders characterized by progressive degeneration of the cerebellum, brain stem and spinal cord. Clinically, ADCA has been divided into three groups: ADCA types I-III. ADCAI is genetically heterogeneous, with five genetic loci, designated spinocerebellar ataxia (SCA) 1, 2, 3, 4 and 6, being assigned to five different chromosomes. ADCAII, which always presents with retinal degeneration (SCA7), and ADCAIII often referred to as the `pure cerebellar syndrome (SCA5), are most likely homogeneous disorders. Several SCA genes have been cloned and shown to contain CAG repeats in their coding regions. ADCA is caused by the expansion of the CAG repeats, producing an elongated polyglutamine tract in the corresponding protein. The expanded repeats are variable in size and unstable, usually increasing in size when transmitted to successive generations. This locus has been mapped to chromosome 3, and it has been determined that the diseased allele associated with Spinocerebellar ataxia-7, contains 38-130 CAG repeats (near the N-terminus), compared to 7-17 in the normal allele. The exact function of this gene is not known, however, since the encoded protein contains a nuclear localization sequence, and is found to be localized in the nucleus, it has been postulated to be a potential transcription factor. Alternative splicing, resulting in transcript variants encoding different isoforms, has been noted for this gene. |
| Gene Symbol |
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| Purification Method | Unpurified |
| UniProt Number |
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| UniProt Summary | UniProtKB/Swiss-Prot: ATX7_HUMAN, O15265 (See protein sequence) Recommended Name: Ataxin-7 Size: 892 amino acids; 95451 Da Subunit: Component of the STAGA transcription coactivator-HAT complex, at least composed of SUPT3H, GCN5L2, TAF5L, TAF6L, SUPT7L, TADA3L, TAD1L, TAF10, TAF12, TRRAP, TAF9 and ATXN7. The STAGA core complex is associated with a subcomplex required for histone deubiquitinylation composed of ATXN7L3, ENY2 and USP22. Interacts with SORBS1, PSMC1 and CRX. Interacts with TRRAP, GCN5L2 and TAF10 Subcellular location: Nucleus. Nucleus, nucleolus. Nucleus matrix. Note=In addition to a diffuse distribution throughout the nucleus, it is associated with the nuclear matrix and the nucleolus Subcellular location: Isoform b: Cytoplasm Secondary accessions: O75328 O75329 Q9Y6P8 Alternative splicing: 2 isoforms: O15265-1 O15265-2 |
| Molecular Weight | 96 kDa Calculated |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting of Rat brain lysate. Western Blotting: Representative lot data: A 1:1000 dilution of this antibody detected Ataxin-7 in 10ug of Rat brain tissue lysate. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. |
| Packaging Information | |
|---|---|
| Material Size | 100 µL |