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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M, R | WB, IP, ICC | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | 04-1624 |
| Description | Anti-Centrin Antibody, clone 20H5 |
| Alternate Names |
|
| Background Information | Centrin is a key regulator of centriole duplication. This protein is localized to the centrosome of interphase cells. During mitosis, centrin redistributes to the spindle poles, consistent with dynamic changes of the centrosome during the cell cycle. Current research suggests centrin exerts spatial and temporal control over centriole duplication through its role as a component of centriole precursor structures and through periodic cell-cycle specific changes in its abundance. |
| Product Information | |
|---|---|
| Format | Purified |
| Control |
|
| Presentation | Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-Centrin Antibody, clone 20H5 is a Mouse Monoclonal Antibody for detection of Centrin also known as Caltractin isoform 2, EF-hand protein & has been validated in WB, IP, ICC. |
| Key Applications |
|
| Application Notes | Immunocytochemistry Analysis: Manandhar, G., et al. (1999). Cell Motil Cytoskeleton. 43(2):137-144. |
| Biological Information | |
|---|---|
| Immunogen | Recombinant protein corresponding to the C-terminus of Chlamydomonas Centrin. |
| Epitope | C-terminus |
| Clone | 20H5 |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Mouse |
| Specificity | This antibody recognizes the C-terminus of Centrin. |
| Isotype | IgG2aκ |
| Species Reactivity |
|
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Entrez Gene Summary | The protein encoded by this gene plays important roles in the determination of centrosome position and segregation, and in the process of microtubule severing. This encoded protein is localized to the centrosome of interphase cells, and redistributes to the region of the spindle poles during mitosis, reflecting the dynamic behavior of the centrosome during the cell cycle. |
| Gene Symbol |
|
| Purification Method | Protein G Purified |
| UniProt Number |
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| UniProt Summary | FUNCTION: Plays a fundamental role in microtubule-organizing center structure and function. SUBUNIT STRUCTURE: Monomer By similarity. SUBCELLULAR LOCATION: Cytoplasm › cytoskeleton › centrosome. Note: Centrosome of interphase and mitotic cells. MISCELLANEOUS: Binds two moles of calcium per mole of protein. SEQUENCE SIMLARITIES: Belongs to the centrin family. Contains 4 EF-hand domains. |
| Molecular Weight | ~ 20 kDa |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blot in HeLa cell lysate. Western Blot Analysis: 1 µg/ml of this antibody detected Centrin on 10 µg of HeLa cell lysate. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |