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Millipore This Anti-Ubiquitinylated Proteins Antibody, Clone Fk2 Has Been Published And Validated For Use In Elisa, Immunofluorescence (If), Immunoprecipitation (Ip) And Western Blotting - Mill (Additional S&H or Hazmat Fees May Apply)
Ubiquitin exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked: Lys-6-linked may be involved in DNA repair; Lys-11-linked is involved in ERAD (endoplasmic reticulum-associated degradation) and in cell-cycle regulation; Lys-29-linked is involved in lysosomal degradation; Lys-33-linked is involved in kinase modification; Lys-48-linked is involved in protein degradation via the proteasome; Lys-63-linked is involved in endocytosis, DNA-damage responses as well as in signaling processes leading to activation of the transcription factor NF-kappa-B. Linear polymer chains formed via attachment by the initiator Met lead to cell signaling. Ubiquitin is usually conjugated to Lys residues of target proteins, however, in rare cases, conjugation to Cys or Ser residues has been observed. When polyubiquitin is free (unanchored-polyubiquitin), it also has distinct roles, such as in activation of protein kinases, and in signaling. Similarity: Belongs to the ubiquitin family. Contains 3 ubiquitin-like domains.
Product Information
Format
Purified
Presentation
100 µL of purified immunoglobulin in PBS containing 0.1% sodium azide. Dilute to working strength with phosphate buffered saline pH 7.2-7.4.
Quality Level
MQ100
Applications
Application
This Anti-Ubiquitinylated proteins Antibody, clone FK2 has been published and validated for use in ELISA, Immunofluorescence (IF), Immunoprecipitation (IP) and Western Blotting (WB) for the detection of Ubiquitinylated proteins.
Key Applications
ELISA
Immunofluorescence
Immunoprecipitation
Western Blotting
Biological Information
Immunogen
Poly-ubiquitinylated-lysozyme.
Clone
clone FK2
Concentration
Please refer to the Certificate of Analysis for the lot-specific concentration.
Host
Mouse
Specificity
Recognizes mono- and poly ubiquitinylated proteins, but not free ubiquitin.
Isotype
IgG1
Species Reactivity
All
Antibody Type
Monoclonal Antibody
Product Usage Statements
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Stable for 1 year at -20°C from date of shipment for up to 12 months.
