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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M, R, B | IP, WB | Rb | Culture Supernatant | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | 04-841 |
| Replaces | 05-841 |
| Description | Anti-ROKα/ROCK-II Antibody, clone A9W4, rabbit monoclonal |
| Alternate Names |
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| Background Information | Rho-Associated Kinase (Rho-Kinase or ROK) has been classified into two isoforms: ROKα/ROCK-II and ROKβ/ROCK-I. These large cellular proteins (150-160 kDa), have been reported to be involved in the formation of stress fibers, focal adhesions and cardiac hypertonic responses. Current research suggests that ROK of smooth muscle may be involved in the contractile process via phosphorylation of MYPT1 and Myosin. |
| Product Information | |
|---|---|
| Format | Culture Supernatant |
| Control |
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| Presentation | Cultured supernatant containing 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Detect ROKα/ROCK-II using this Anti-ROKα/ROCK-II Antibody, clone A9W4 validated for use in IP & WB. |
| Key Applications |
|
| Application Notes | Immunoprecipitation: 4 μL of a previous lot immunoprecipitated ROKα/ROCK-II from 500 μg of PC-12 lysate. |
| Biological Information | |
|---|---|
| Immunogen | 6His-tagged fusion protein corresponding to residues 2-543 of rat ROKα/ROCK-II. |
| Clone | A9W4 |
| Host | Rabbit |
| Specificity | Recognizes ROKα/ROCK-II, Mr 150 kDa. Does not recognize ROKβ/ROCK-I. A non-specific band may also be detected, Mr 80 kDa. |
| Species Reactivity |
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| Species Reactivity Note | Human and rat. Predicted to cross-react with mouse and bovine based on sequence homology. |
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Entrez Gene Summary | A Rho kinase that is involved in inducing membrane blebbing and chromatin condensation [RGD]. |
| Gene Symbol |
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| UniProt Number |
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| UniProt Summary | FUNCTION: Protein kinase that phosphorylates a large number of important signaling proteins, and thereby regulates the assembly of the actin cytoskeleton, cell migration, invasiveness of tumor cells, smooth muscle contraction and neurite outgrowth. Necessary for apoptotic membrane blebbing. Plays a role in smooth muscle contraction. Required for centromere positioning and centromere-dependent exit from mitosis By similarity. Catalytic activity ATP + a protein = ADP + a phosphoprotein. Enzyme regulation Activated by RHOA binding. SUBUNIT: Binds RHOA that has been activated by GTP binding. Binds ADD1, GEM, RHOB, RHOC, myosin light chain and VIM By similarity. Binds RHOE, PPP1R12A (MYPT1/MBS), LIMK1 and LIMK2. SUBCELLULAR LOCATION: Cytoplasm. Centrosome › centrioleBy similarity. Golgi apparatus membrane; Peripheral membrane protein. Note= Associated with the mother centriole and an intercentriolar linker By similarity. A small proportion is associated with Golgi membranes. TISSUE SPECIFICITY: Detected in blood platelets. Ref.1 DOMAIN: The C-terminal auto-inhibitory domain interferes with kinase activity. RHOA binding leads to a conformation change and activation of the kinase. Truncated ROCK1 is constitutively activated. PTM: Autophosphorylated on serine and threonine residues. Phosphorylated upon DNA damage, probably by ATM or ATR. Ref.1 Ref.10 Ref.11 Ref.12 Cleaved by caspase-3 during apoptosis. This leads to constitutive activation of the kinase and membrane blebbing. Miscellaneous Inhibited by Y-27632. SIMILARITY: Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. Contains 1 AGC-kinase C-terminal domain. Contains 1 PH domain. Contains 1 phorbol-ester/DAG-type zinc finger. Contains 1 protein kinase domain. Contains 1 REM (Hr1) repeat. |
| Molecular Weight | 150 kDa |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Routinely evaluated by Western BLot on RIPA lysates from PC-12 cells. Western Blot Analysis: A 1:500-1:5000 dilution of this antibody detected ROKα/ROCK-II in RIPA lysates from PC-12 cells. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at -20ºC from date of receipt. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. |
| Packaging Information | |
|---|---|
| Material Size | 200 µL |