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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| Vrt, M | WB | Rb | Culture Supernatant | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | 04-857 |
| Replaces | 05-857 |
| Brand Family | Upstate |
| Trade Name |
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| Description | Anti-phospho-Src family (Tyr416) Antibody, clone 2N8, rabbit monoclonal |
| Alternate Names |
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| Background Information | Src (also known as pp60src) is a non receptor Tyrosine Kinase involved in signal transduction in many biological systems and implicated in the development of human tumors. There are two critical phosphorylation sites of tyrosine on Src, tyrosine 418 and tyrosine 529 (referring to human Src sequence). The tyrosine 418 is located in the catalytic domain and is one of the autophosphorylation sites. Full catalytic activity of Src requires phosphorylation of tyrosine 418. This region of Src is also highly conserved in all of the related Src-family kinases, and thus prior immunoprecipitation may be required to identify which Src family member is being activated. |
| Product Information | |
|---|---|
| Format | Culture Supernatant |
| Control |
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| Presentation | Cultured supernantant in 0.05% sodium azide |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-phospho-Src family (Tyr416) Antibody, clone 2N8 is an antibody against phospho-Src family (Tyr416) for use in WB. |
| Key Applications |
|
| Application Notes | Western Blot Analysis: A 1:1000 dilution of this lot detected phospho-c-Src in RIPA lysates from COS cells transfected with active Src (Cat # 21-115) (Figure A). Immunoprecipitation: A previous lot detected endogenous phospho-c-Src immunoprecipitated from NIH/3T3 RIPA lysates with anti-Src, clone GD11 agarose beads (Cat # 16-186) (Figure B). |
| Biological Information | |
|---|---|
| Immunogen | KLH-conjugated, synthetic peptide containing the sequence ...NE[pY]TA…, where pY corresponds to phosphotyrosine at position 416 of c-Src |
| Clone | 2N8 |
| Host | Rabbit |
| Specificity | phospho-c-Src (Tyr416) |
| Isotype | IgG |
| Species Reactivity |
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| Species Reactivity Note | Mouse. Broad species cross-reactivity expected based on sequence homology. |
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Entrez Gene Summary | This gene is highly similar to the v-src gene of Rous sarcoma virus. This proto-oncogene may play a role in the regulation of embryonic development and cell growth. The protein encoded by this gene is a tyrosine-protein kinase whose activity can be inhibited by phosphorylation by c-SRC kinase. Mutations in this gene could be involved in the malignant progression of colon cancer. Two transcript variants encoding the same protein have been found for this gene. |
| Gene Symbol |
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| Modifications |
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| UniProt Number |
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| UniProt Summary | SIZE: 536 amino acids; 59835 Da SUBUNIT: Interacts with DDEF1/ASAP1 via its SH3 domain. Interacts with CCPG1 (By similarity). Interacts with CDCP1, PELP1, TGFB1I1 and TOM1L2. PTM: Phosphorylated on Tyr-530 by c-Src kinase (CSK). The phosphorylated form is termed pp60c-src. The phosphorylated tail interacts with the SH2 domain thereby repressing kinase activity. SIMILARITY: SwissProt: P12931 ## Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily. & Contains 1 protein kinase domain. & Contains 1 SH2 domain. & Contains 1 SH3 domain. |
| Molecular Weight | 60kDa |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Routinely evaluated by immunoblot of endogenous phospho-c-Src immunoprecipitated from NIH/3T3 RIPA lysates with anti-Src, clone GD11 agarose beads (Cat # 16-186) |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. |
| Packaging Information | |
|---|---|
| Material Size | 100 µL |