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Millipore This Anti-Pdgfr Antibody, Clone Ch-3 Is Validated For Use In Wb, Elisa, Ih(P) For The Detection Of Pdgfr. - Mill (Additional S&H or Hazmat Fees May Apply)
PDGFR (Platelet-Derived Growth Factor Receptor) is a receptor tyrosine kinase for the PDGF family of growth factors. PDGF homo and hetero- dimmers (dimer of A (17 kDa) and B (14 kDa) chains (AA, AB, or BB)) bind to the two structurally related a and b PDGF Receptors (PDGFRa and PDGFRb). The type A PDGF Receptor binds all three PDGFs with high affinity, whereas the type B PDGF Receptor is selective for PDGF-BB. This binding results in the dimerization of the receptor and the subsequent kinase activation of the receptor tyrosine kinase (RTK) and its autophosphorylation. These phosphorylations allow for various proteins, such as PI3 Kinase, PLCg, and Grb2, to bind through their SH2 domains and transduce signals into the cell. The activation of PDGFFR triggers the intracellular loop and activates Ras, Erk1/2, and ROS. PDGFb has been implicated in embryonic development, angiogenesis, and development of tumors of glial origin.
Product Information
Format
Purified
Control
Jurkat cell lysate
Presentation
Purified mouse monoclonal IgG1 in buffer containing 20 mM sodium phosphate, 250 mM NaCl, 0.1% NaN3, pH. 7.6
Quality Level
MQ100
Applications
Application
This Anti-PDGFR Antibody, clone CH-3 is validated for use in WB, ELISA, IH(P) for the detection of PDGFR.
Key Applications
Western Blotting
ELISA
Immunohistochemistry (Paraffin)
Application Notes
ELISA: A previous lot of this antibody was used in ELISA.
Immunohistochemistry(paraffin): Representative images from a previous lot. Optimal Staining of PDGF-A With Citrate pH 6.0 Epitope Retrieval: Rat Brain
Please refer to the Certificate of Analysis for the lot-specific concentration.
Host
Mouse
Isotype
IgG1
Species Reactivity
Human
Mouse
Rat
Species Reactivity Note
Human and mouse. Expected to react with rat based on sequence homology.
Antibody Type
Monoclonal Antibody
Purification Method
Protein A Purfied
UniProt Number
P09619
Molecular Weight
211 amino acids; 24043 Da
Product Usage Statements
Quality Assurance
Routinely evaluated by Western Blot on Jurkat lysates.
Western Blot Analysis: 1:500 dilution of this lot detected PDGFR on 10 μg of Jurkat lysates.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Maintain at + 2-8 ° C in undiluted aliquots for up to 1 year from date of receipt.
