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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | WB | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | 05-1143 |
| Replaces | MAB1146 |
| Description | Anti-phospho-Paxillin (Tyr31) Antibody, clone M102 |
| Background Information | Paxillin is involved in focal adhesion formation during cell adhesion and migration. Paxillin contains LD motifs, LIM domains, and an SH3- and SH2-binding domain that participate in a variety of protein-protein interactions with kinases, GTPase-activating proteins, and cytoskeletal proteins. Phosphorylation of paxillin occurs at both tyrosine and serine sites. Tyrosine phosphorylation of paxillin occurs in response to growth factors, neuropeptides, and integrins. The major sites of tyrosine phosphorylation include Tyr31 and Tyr118. Both of these sites may be involved in Crk binding to paxillin during integrin-mediated cell adhesion. These sites may provide docking motifs for recruitment of other signaling molecules to focal adhesions. |
| Product Information | |
|---|---|
| Format | Purified |
| Control |
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| Presentation | Purified monoclonal IgG1 in phosphate-buffered saline, 50% glycerol, 1 mg/mL BSA, and 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Detect phospho-Paxillin (Tyr31) using this Anti-phospho-Paxillin (Tyr31) Antibody, clone M102 validated for use in WB. |
| Key Applications |
|
| Biological Information | |
|---|---|
| Immunogen | Clone M102 was generated from phospho-Paxillin (Tyr31) synthetic peptide (coupled to KLH) corresponding to amino acid residues around tyrosine 31 of human paxillin. This human sequence is highly conserved in rat and mouse paxillin. |
| Clone | M102 |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Mouse |
| Specificity | The antibody detects Paxillin phosphorylated at Tyr31. |
| Isotype | IgG1 |
| Species Reactivity |
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| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Modifications |
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| Purification Method | Protein A chromatography |
| UniProt Number |
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| UniProt Summary | FUNCTION: Cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion). SUBUNIT STRUCTURE: Binds in vitro to vinculin as well as to the SH3 domain of c-SRC and, when tyrosine phosphorylated, to the SH2 domain of V-CRK. Isoform beta binds to focal adhesion kinase but weakly to vinculin. Isoform gamma binds to vinculin but only weakly to focal adhesion kinase. Interacts with GIT1, NUDT16L1/SDOS, PARVA and TGFB1I1. Component of cytoplasmic complexes, which also contain GIT1, ARHGEF6 and PAK1 By similarity. Binds ASAP2. Interacts with unphosphorylated ITGA4. Interacts with RNF5. SUBCELLULAR LOCATION: Cytoplasm › cytoskeleton. Cell junction › focal adhesion. PTM: Phosphorylated on tyrosine residues during integrin-mediated cell adhesion, embryonic development, fibroblast transformation and following stimulation of cells by mitogens. SEQUENCE SIMILARITIES: Belongs to the paxillin family. Contains 4 LIM zinc-binding domains. |
| Molecular Weight | 72 kDa |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by western Blot in pervanadate treated L6 cell lysates. Western Blot Analysis: 1:500 dilution of this lot detected phospho-Paxillin on 10 µg of pervanadate treated L6 lysates. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Maintain at -20°C for one year from date of receipt. Do not aliquot. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage. |
| Packaging Information | |
|---|---|
| Material Size | 100 µL |