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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| B, H, M, R | NEUT, RIA | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | 05-117 |
| Brand Family | Upstate |
| Trade Name |
|
| Description | Anti-FGF-2/basic FGF (neutralizing) Antibody, clone bFM-1 |
| Alternate Names |
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| Background Information | Basic fibroblast growth factor, also known as bFGF or FGF2, is a member of the fibroblast growth factor family. In normal tissue, basic fibroblast growth factor is present in basement membranes and in the subendothelial extracellular matrix of blood vessels. It stays membrane-bound as long as there is no signal peptide. It has been hypothesized that, during both wound healing of normal tissues and tumor development, the action of heparan sulfate-degrading enzymes activates bFGF, thus mediating the formation of new blood vessels, a process known as angiogenesis. Additionally, bFGF is a critical component of human embryonic stem cell culture medium; the growth factor is necessary for the cells to remain in an undifferentiated state, although the mechanisms by which it does this are poorly defined. |
| Product Information | |
|---|---|
| Format | Purified |
| Control |
|
| Presentation | Purified mouse monoclonal IgG1κ in TBS. Protein determined by a Bradford microtiter assay using mouse IgG as the standard. Frozen solution. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-FGF-2/basic FGF (neutralizing) Antibody, clone bFM-1 is an antibody against FGF-2/basic FGF (neutralizing) for use in NEUT, RIA. |
| Key Applications |
|
| Applications Not Recommended |
|
| Biological Information | |
|---|---|
| Immunogen | Purified bovine brain basic FGF (HBGF-2). Clone bFM-1. |
| Epitope | a.a. 5-24 |
| Clone | bFM-1 |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Mouse |
| Specificity | This antibody recognizes the non-denatured form of FGF-2/basic FGF; does not cross-react with bovine acidic FGF. |
| Isotype | IgG1κ |
| Species Reactivity |
|
| Species Reactivity Note | This antibody is highly specific for the non-denatured form of FGF-2/basic FGF from bovine, human, rat and mouse sources. Does not cross-react with bovine acidic FGF. |
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
|
| Entrez Gene Summary | The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members bind heparin and possess broad mitogenic and angiogenic activities. This protein has been implicated in diverse biological processes, such as limb and nervous system development, wound healing, and tumor growth. The mRNA for this gene contains multiple polyadenylation sites, and is alternatively translated from non-AUG (CUG) and AUG initiation codons, resulting in five different isoforms with distinct properties. The CUG-initiated isoforms are localized in the nucleus and are responsible for the intracrine effect, whereas, the AUG-initiated form is mostly cytosolic and is responsible for the paracrine and autocrine effects of this FGF. |
| Gene Symbol |
|
| Purification Method | Ammonium sulfate precipitation and DEAE-cellulose chromatography |
| UniProt Number |
|
| UniProt Summary | FUNCTION: SwissProt: P09038 # The heparin-binding growth factors are angiogenic agents in vivo and are potent mitogens for a variety of cell types in vitro. There are differences in the tissue distribution and concentration of these 2 growth factors. SIZE: 155 amino acids; 17254 Da SUBUNIT: Monomer. Interacts with CSPG4 and FGFBP1. Found in a complex with FGFBP1, FGF1 and FGF2. SIMILARITY: SwissProt: P09038 ## Belongs to the heparin-binding growth factors family. MISCELLANEOUS: This protein binds heparin more strongly than does aFGF. ... hide » see more » |
| Molecular Weight | 17.5 kDa |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Routinely evaluated by neutralization of greater than 50% of growth of FBHE cells simulated with FGF-2/bFGF. Neutralization of bFGF Activity Assay: 2.5 µg/mL of this lot of antibody neutralized greater than 40% of growth of FBHE cells simulated with 5 ng/mL of FGF-2/bFGF. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at -20ºC from date of receipt. Aliquot to avoid repeated freezing and thawing. For maximum recovery of the product, centrifuge the original vial after thawing and prior to removing the cap. |
| Packaging Information | |
|---|---|
| Material Size | 500 µg |